M/Z: 459.2349
Hit 5 annotations: Flumethasone Pivalate_[M+H-2H2O]+; Hydroxystrobilurin D_[M+H]+; Cinobufotalin_[M+H]+; Ustiloxin D_[M+H-2H2O]+; Retinoyl b-glucuronide_[M+H-H2O]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 8 Reference Ions Near m/z 459.2349
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000045979 Reliable | 459.2351 | 459.2351 ~ 459.2351 MzDiff: none |
Hydroxystrobilurin D (BioDeep_00000034697) Formula: C26H34O7 (458.2304) |
7.34 (100%) | Mus musculus [UBERON:0002107] liver |
| MSI_000002583 Unreliable | 459.2305 | 459.2305 ~ 459.2305 MzDiff: none |
Flumethasone Pivalate (BioDeep_00000026769) Formula: C27H36F2O6 (494.248) |
0.25 (100%) | Rattus norvegicus [UBERON:0001950] neocortex |
| MSI_000003562 Unavailable | 459.2305 | 459.2305 ~ 459.2305 MzDiff: none |
Flumethasone Pivalate (BioDeep_00000026769) Formula: C27H36F2O6 (494.248) |
-0.61 (100%) | Rattus norvegicus [UBERON:0002037] cerebellum |
| MSI_000004832 Unreliable | 459.2305 | 459.2305 ~ 459.2305 MzDiff: none |
Flumethasone Pivalate (BioDeep_00000026769) Formula: C27H36F2O6 (494.248) |
1.35 (100%) | Rattus norvegicus [UBERON:0002298] brainstem |
| MSI_000006038 Unavailable | 459.2305 | 459.2305 ~ 459.2305 MzDiff: none |
Flumethasone Pivalate (BioDeep_00000026769) Formula: C27H36F2O6 (494.248) |
-1.83 (100%) | Rattus norvegicus [UBERON:0002435] striatum |
| MSI_000058736 Unreliable | 459.2274 | 459.2274 ~ 459.2274 MzDiff: none |
Ustiloxin D (BioDeep_00000025886) Formula: C23H34N4O8 (494.2377) |
1.02 (100%) | Mus musculus [UBERON:0001950] neocortex |
| MSI_000060116 Unavailable | 459.2274 | 459.2274 ~ 459.2274 MzDiff: none |
Ustiloxin D (BioDeep_00000025886) Formula: C23H34N4O8 (494.2377) |
-0.98 (100%) | Mus musculus [UBERON:0002298] brainstem |
| MSI_000060412 Unavailable | 459.2274 | 459.2274 ~ 459.2274 MzDiff: none |
Ustiloxin D (BioDeep_00000025886) Formula: C23H34N4O8 (494.2377) |
-0.04 (100%) | Mus musculus [UBERON:0002421] hippocampal formation |
Found 7 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| Flumethasone Pivalate Formula: C27H36F2O6 (494.248) Adducts: [M+H-2H2O]+ (Ppm: 8.3) |
Rattus norvegicus (Brain) |
Spectroswiss - sol_2x_br_2Resolution: 17μm, 488x193
|
|
| Hydroxystrobilurin D Formula: C26H34O7 (458.2304) Adducts: [M+H]+ (Ppm: 10.9) |
Macropus giganteus (Brain) |
170321_kangaroobrain-dan3-pos_maxof50.0_med1Resolution: 50μm, 81x50
Sample information
Organism: Macropus giganteus (kangaroo)
Organism part: Brain
Condition: Wildtype
Sample growth conditions: Wild |
|
| Hydroxystrobilurin D Formula: C26H34O7 (458.2304) Adducts: [M+H]+ (Ppm: 6.1) |
Homo sapiens (esophagus) |
LNTO22_1_4Resolution: 17μm, 82x80
|
|
| Hydroxystrobilurin D Formula: C26H34O7 (458.2304) Adducts: [M+H]+ (Ppm: 5.7) |
Mus musculus (Liver) |
Salmonella_final_pos_recalResolution: 17μm, 691x430
A more complete and holistic view on host–microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium.
[dataset] Nicole Strittmatter. Holistic Characterization of a Salmonella Typhimurium Infection Model Using Integrated Molecular Imaging, metabolights_dataset, V1; 2022. https://www.ebi.ac.uk/metabolights/MTBLS2671. |
|
| Cinobufotalin Formula: C26H34O7 (458.2304) Adducts: [M+H]+ (Ppm: 11.4) |
Mytilus edulis (mantle) |
20190216_MS38_Mytilus_mantle_350-1500_DHB_pos_A26_10um_275x210Resolution: 10μm, 275x210
|
|
| Ustiloxin D Formula: C23H34N4O8 (494.2377) Adducts: [M+H-2H2O]+ (Ppm: 7.8) |
Mus musculus (brain) |
Brain01_Bregma-3-88b_centroidResolution: 17μm, 265x320
|
|
| Retinoyl b-glucuronide Formula: C26H36O8 (476.241) Adducts: [M+H-H2O]+ (Ppm: 19.9) |
Drosophila melanogaster (brain) |
Drosophila18Resolution: 5μm, 686x685
Sample information
Organism: Drosophila melanogaster
Organism part: Brain
Condition: Healthy
Sample preparation
Sample stabilisation: Frozen
Tissue modification: Frozen
MALDI matrix: 2,5-dihydroxybenzoic acid (DHB)
MALDI matrix application: TM sprayer
Solvent: Aceton/water
MS analysis
Polarity: Positive
Ionisation source: Prototype
Analyzer: Orbitrap
Pixel size: 5μm × 5μm
Annotation settings
m/z tolerance (ppm): 3
Analysis version: Original MSM
Pixel count: 469910
Imzml file size: 696.23 MB
Ibd file size: 814.11 MB |
|
