- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 13 Reference Ions Near m/z 681.9931
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000063859 Reliable | 681.991 | 681.991 ~ 681.991 MzDiff: none |
Not Annotated | (%) | Homo sapiens [UBERON:0001264] pancreas |
| MSI_000027322 Unreliable | 681.9898 | 681.9898 ~ 681.9898 MzDiff: none |
Perfluorotridecanoic acid (BioDeep_00000182267) Formula: C13HF25O2 (663.9577) |
2.03 (100%) | Mus musculus [UBERON:0000913] interstitial fluid |
| MSI_000001227 Unavailable | 681.9931 | 681.9931 ~ 681.9931 MzDiff: none |
Perfluorotridecanoic acid (BioDeep_00000182267) Formula: C13HF25O2 (663.9577) |
-0.5 (100%) | Mus musculus [UBERON:0001224] renal pelvis |
| MSI_000001675 Unavailable | 681.9931 | 681.9931 ~ 681.9931 MzDiff: none |
Perfluorotridecanoic acid (BioDeep_00000182267) Formula: C13HF25O2 (663.9577) |
-0.5 (100%) | Mus musculus [UBERON:0001225] cortex of kidney |
| MSI_000002143 Unavailable | 681.9931 | 681.9931 ~ 681.9931 MzDiff: none |
Perfluorotridecanoic acid (BioDeep_00000182267) Formula: C13HF25O2 (663.9577) |
-0.49 (100%) | Mus musculus [UBERON:0001293] outer medulla of kidney |
| MSI_000027338 Unreliable | 681.9973 | 681.9973 ~ 681.9973 MzDiff: none |
Not Annotated | 2.03 (%) | Mus musculus [UBERON:0000913] interstitial fluid |
| MSI_000001429 Unavailable | 681.9998 | 681.9998 ~ 681.9998 MzDiff: none |
Not Annotated | -0.71 (%) | Mus musculus [UBERON:0001224] renal pelvis |
| MSI_000001881 Unavailable | 681.9998 | 681.9998 ~ 681.9998 MzDiff: none |
Not Annotated | -0.71 (%) | Mus musculus [UBERON:0001225] cortex of kidney |
| MSI_000002302 Unavailable | 681.9998 | 681.9998 ~ 681.9998 MzDiff: none |
Not Annotated | -0.64 (%) | Mus musculus [UBERON:0001293] outer medulla of kidney |
| MSI_000002989 Unavailable | 682.0029 | 682.0029 ~ 682.0029 MzDiff: none |
Amiodarone HCl (BioDeep_00000399012) Formula: C25H30ClI2NO3 (681.0004) |
-0.78 (100%) | Rattus norvegicus [UBERON:0001950] neocortex |
| MSI_000003600 Unavailable | 682.0029 | 682.0029 ~ 682.0029 MzDiff: none |
Amiodarone HCl (BioDeep_00000399012) Formula: C25H30ClI2NO3 (681.0004) |
-0.67 (100%) | Rattus norvegicus [UBERON:0002037] cerebellum |
| MSI_000005244 Unavailable | 682.0029 | 682.0029 ~ 682.0029 MzDiff: none |
Amiodarone HCl (BioDeep_00000399012) Formula: C25H30ClI2NO3 (681.0004) |
-0.79 (100%) | Rattus norvegicus [UBERON:0002298] brainstem |
| MSI_000005554 Unreliable | 682.0029 | 682.0029 ~ 682.0029 MzDiff: none |
Amiodarone HCl (BioDeep_00000399012) Formula: C25H30ClI2NO3 (681.0004) |
0.92 (100%) | Rattus norvegicus [UBERON:0002435] striatum |
Found 5 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| Perfluorotridecanoic acid Formula: C13HF25O2 (663.9577) Adducts: [M+NH4]+ (Ppm: 2.3) |
Mus musculus (Kidney) |
FULL_MS_centriod_CHCA_20210819Resolution: 17μm, 638x437
AP-MALDI instrument demo test, mass spectrum scan in centroid mode. |
|
| Perfluorotridecanoic acid Formula: C13HF25O2 (663.9577) Adducts: [M+NH4]+ (Ppm: 2.6) |
Mus musculus (Lung) |
image2Resolution: 40μm, 550x256
Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b)
[PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9-
choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in
parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in
white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar
regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids
PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using
total ion current normalisation and hotspot removal (high quantile = 99%). |
|
| m/z_681.9973 Formula: - (n/a) Adducts: (Ppm: ) |
Mus musculus (Lung) |
image2Resolution: 40μm, 550x256
Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b)
[PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9-
choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in
parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in
white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar
regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids
PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using
total ion current normalisation and hotspot removal (high quantile = 99%). |
|
| Perfluorotridecanoic acid Formula: C13HF25O2 (663.9577) Adducts: [M+NH4]+ (Ppm: 2.7) |
Mus musculus (brain) |
Brain02_Bregma-3-88Resolution: 17μm, 288x282
|
|
| m/z_681.991 Formula: - (n/a) Adducts: (Ppm: ) |
Homo sapiens (Pancreas) |
tma6Resolution: 17μm, 368x255
Sample information
Organism: Homo sapiens (human)
Organism part: Pancreas
Condition: N/A
Sample preparation
Sample stabilisation: Paraformaldehyde fixed
Tissue modification: None
MALDI matrix: CHCA
MALDI matrix application: quantification
Solvent: none
MS analysis
Polarity: Positive
Ionisation source: MALDI
Analyzer: timsTOF fleX
Pixel size: 0.1μm × 0.1μm
Annotation settings
m/z tolerance (ppm): 3
Analysis version: Original MSM
Pixel count: 43035
Imzml file size: 94.45 MB
Ibd file size: 2.41 GB |
|
