在BioDeep NovoCell知识数据库中,参考离子总共被划分为4个级别。
  • Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
  • Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
  • Unreliable: 这个参考离子具有较高的排名价值,但缺乏可重复性。
  • Unavailable: 由于排名价值低且缺乏可重复性,这个参考离子不应用于注释。

Found 25 Reference Ions Near m/z 327.0165
NovoCell ID m/z Mass Window Metabolite Ranking Anatomy Context
MSI_000054418 Reliable 327.0084 327.008 ~ 327.0087
MzDiff: 2.8 ppm
4-bromo-n-[3-(2,5-dihydroxyimidazol-4-ylidene)propyl]-1h-pyrrole-2-carboxamide (BioDeep_00002178580)
Formula: C11H11BrN4O3 (326.0014)
2.5 (100%) MALDI - CHCA
[NOVOCELL:BACKGROUND] blank
MSI_000054136 Reliable 327.0165 327.0163 ~ 327.0167
MzDiff: 1.8 ppm
3-(1-bromopropylidene)-6-chloro-5-(pent-2-en-4-yn-1-yl)-4,9-dioxabicyclo[6.1.0]nonane (BioDeep_00002228807)
Formula: C15H18BrClO2 (344.0179)
3.36 (50%) MALDI - CHCA
[NOVOCELL:BACKGROUND] blank
MSI_000015352 Reliable 327.0243 327.0242 ~ 327.0244
MzDiff: 0.5 ppm
8-(1-bromoprop-1-en-1-yl)-6-hydroxy-4,4-dimethyl-5-oxatricyclo[4.4.0.0¹,³]dec-8-ene-7,10-dione (BioDeep_00002139522)
Formula: C14H15BrO4 (326.0154)
5.18 (100%) Vitis vinifera
[PO:0009085] exocarp
MSI_000032147 Unreliable 327.0263 327.0261 ~ 327.0265
MzDiff: 1.6 ppm
galop; malic acid (BioDeep_00002094119)
Formula: C11H12O10 (304.043)
5.95 (67%) Posidonia oceanica
[PO:0005020] vascular bundle
MSI_000013066 Unavailable 327.0166 327.0166 ~ 327.0166
MzDiff: none
Diclofop (BioDeep_00000012392)
Formula: C15H12Cl2O4 (326.0113)
-0.66 (100%) Plant
[PO:0005020] vascular bundle
MSI_000013234 Unavailable 327.009 327.009 ~ 327.009
MzDiff: none
methoxychlor (BioDeep_00000008459)
Formula: C16H15Cl3O2 (344.0138)
-0.87 (100%) Plant
[PO:0005020] vascular bundle
MSI_000013572 Unreliable 327.009 327.009 ~ 327.009
MzDiff: none
methoxychlor (BioDeep_00000008459)
Formula: C16H15Cl3O2 (344.0138)
0.73 (100%) Plant
[PO:0005417] phloem
MSI_000013594 Unreliable 327.0262 327.0262 ~ 327.0262
MzDiff: none
Diloxanide (BioDeep_00000006557)
Formula: C14H11Cl2NO4 (327.0065)
0.66 (100%) Plant
[PO:0005417] phloem
MSI_000013776 Unreliable 327.0166 327.0166 ~ 327.0166
MzDiff: none
Diclofop (BioDeep_00000012392)
Formula: C15H12Cl2O4 (326.0113)
0.16 (100%) Plant
[PO:0005417] phloem
MSI_000014695 Unavailable 327.0262 327.0262 ~ 327.0262
MzDiff: none
Diloxanide (BioDeep_00000006557)
Formula: C14H11Cl2NO4 (327.0065)
-0.15 (100%) Plant
[PO:0006036] root epidermis
MSI_000015254 Unavailable 327.0166 327.0166 ~ 327.0166
MzDiff: none
Diclofop (BioDeep_00000012392)
Formula: C15H12Cl2O4 (326.0113)
-0.65 (100%) Plant
[PO:0006036] root epidermis
MSI_000018635 Unreliable 327.0166 327.0166 ~ 327.0166
MzDiff: none
Diclofop (BioDeep_00000012392)
Formula: C15H12Cl2O4 (326.0113)
1.68 (100%) Plant
[PO:0020124] root stele
MSI_000018793 Unreliable 327.009 327.009 ~ 327.009
MzDiff: none
methoxychlor (BioDeep_00000008459)
Formula: C16H15Cl3O2 (344.0138)
1.37 (100%) Plant
[PO:0020124] root stele
MSI_000018920 Unreliable 327.0262 327.0262 ~ 327.0262
MzDiff: none
Diloxanide (BioDeep_00000006557)
Formula: C14H11Cl2NO4 (327.0065)
0.62 (100%) Plant
[PO:0020124] root stele
MSI_000019453 Unreliable 327.0262 327.0262 ~ 327.0262
MzDiff: none
Diloxanide (BioDeep_00000006557)
Formula: C14H11Cl2NO4 (327.0065)
0.55 (100%) Plant
[PO:0025197] stele
MSI_000019802 Unavailable 327.009 327.009 ~ 327.009
MzDiff: none
methoxychlor (BioDeep_00000008459)
Formula: C16H15Cl3O2 (344.0138)
-0.43 (100%) Plant
[PO:0025197] stele
MSI_000020129 Unavailable 327.0166 327.0166 ~ 327.0166
MzDiff: none
Diclofop (BioDeep_00000012392)
Formula: C15H12Cl2O4 (326.0113)
-0.53 (100%) Plant
[PO:0025197] stele
MSI_000032811 Unreliable 327.008 327.008 ~ 327.008
MzDiff: none
4-bromo-n-[3-(2,5-dihydroxyimidazol-4-ylidene)propyl]-1h-pyrrole-2-carboxamide (BioDeep_00002178580)
Formula: C11H11BrN4O3 (326.0014)
0.22 (100%) Posidonia oceanica
[PO:0005020] vascular bundle
MSI_000033606 Unreliable 327.008 327.008 ~ 327.008
MzDiff: none
4-bromo-n-[3-(2,5-dihydroxyimidazol-4-ylidene)propyl]-1h-pyrrole-2-carboxamide (BioDeep_00002178580)
Formula: C11H11BrN4O3 (326.0014)
1.43 (100%) Posidonia oceanica
[PO:0005352] xylem
MSI_000034037 Unreliable 327.0167 327.0167 ~ 327.0167
MzDiff: none
3-(1-bromopropylidene)-6-chloro-5-(pent-2-en-4-yn-1-yl)-4,9-dioxabicyclo[6.1.0]nonane (BioDeep_00002228807)
Formula: C15H18BrClO2 (344.0179)
0.05 (100%) Posidonia oceanica
[PO:0005352] xylem
MSI_000034619 Unreliable 327.0261 327.0261 ~ 327.0261
MzDiff: none
8-(1-bromoprop-1-en-1-yl)-6-hydroxy-4,4-dimethyl-5-oxatricyclo[4.4.0.0¹,³]dec-8-ene-7,10-dione (BioDeep_00002139522)
Formula: C14H15BrO4 (326.0154)
0.43 (100%) Posidonia oceanica
[PO:0006036] root epidermis
MSI_000035561 Unavailable 327.008 327.008 ~ 327.008
MzDiff: none
4-bromo-n-[3-(2,5-dihydroxyimidazol-4-ylidene)propyl]-1h-pyrrole-2-carboxamide (BioDeep_00002178580)
Formula: C11H11BrN4O3 (326.0014)
-0.11 (100%) Posidonia oceanica
[PO:0006203] pericycle
MSI_000038101 Unavailable 327.008 327.008 ~ 327.008
MzDiff: none
4-bromo-n-[3-(2,5-dihydroxyimidazol-4-ylidene)propyl]-1h-pyrrole-2-carboxamide (BioDeep_00002178580)
Formula: C11H11BrN4O3 (326.0014)
-0.3 (100%) Posidonia oceanica
[UBERON:0000329] hair root
MSI_000040027 Unreliable 327.0087 327.0087 ~ 327.0087
MzDiff: none
4-bromo-n-[3-(2,5-dihydroxyimidazol-4-ylidene)propyl]-1h-pyrrole-2-carboxamide (BioDeep_00002178580)
Formula: C11H11BrN4O3 (326.0014)
0.18 (100%) Posidonia oceanica
[PO:0005417] phloem
MSI_000040238 Unavailable 327.0163 327.0163 ~ 327.0163
MzDiff: none
Not Annotated -0.1 (0%) Posidonia oceanica
[PO:0005417] phloem

Found 7 Sample Hits
Metabolite Species Sample
methoxychlor

Formula: C16H15Cl3O2 (344.0138)
Adducts: [M+H-H2O]+ (Ppm: 6.3)
Marker Pen (NA)
3ul_0.8Mpa_RAW_20241016-PAPER PNMK
Resolution: 30μm, 315x42

Description

By writing the four English letters “PNMK” on white paper with a marker pen, and then scanning with a DESI ion source to obtain the scanning result. The signal of the chemical substances on the marker pen used appears on the channel with an m/z value of 322.1918, 323.1953, 546.4010, and etc, from the single cell deconvolution sampling layer class_4. This test data was tested by chuxiaoping from PANOMIX’s R&D laboratory.

Diclofop

Formula: C15H12Cl2O4 (326.0113)
Adducts: [M+H]+ (Ppm: 5.9)
Plant (Root)
MPIMM_035_QE_P_PO_6pm
Resolution: 30μm, 165x170

Description

3-(1-bromopropylidene)-6-chloro-5-(pent-2-en-4-yn-1-yl)-4,9-dioxabicyclo[6.1.0]nonane

Formula: C15H18BrClO2 (344.0179)
Adducts: [M+H-H2O]+ (Ppm: 6.5)
Posidonia oceanica (root)
20190614_MS1_A19r-20
Resolution: 17μm, 262x276

Description

Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth. Despite covering less than 0.1% of coastal regions, they have the capacity to bury up to 10% of marine organic matter and can bury the same amount of carbon 35 times faster than tropical rainforests. On land, the soil’s ability to sequestrate carbon is intimately linked to microbial metabolism. Despite the growing attention to the link between plant production, microbial communities, and the carbon cycle in terrestrial ecosystems, these processes remain enigmatic in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do not fully use this sugar stock in their metabolism. Instead, sucrose piles up in the sediments to mM concentrations underneath multiple types of seagrass meadows. Sediment incubation experiments show that microbial communities living underneath a meadow use sucrose at low metabolic rates. Our metagenomic analyses revealed that the distinct community of microorganisms occurring underneath meadows is limited in their ability to degrade simple sugars, which allows these compounds to persist in the environment over relatively long periods of time. Our findings reveal how seagrasses form blue carbon stocks despite the relatively small area they occupy. Unfortunately, anthropogenic disturbances are threatening the long-term persistence of seagrass meadows. Given that these sediments contain a large stock of sugars that heterotopic bacteria can degrade, it is even more important to protect these ecosystems from degradation.

m/z_327.0146

Formula: - (n/a)
Adducts: (Ppm: 0)
Posidonia oceanica (root)
20190822_MS1_A19r-19
Resolution: 17μm, 303x309

Description

Seagrasses are among the most efficient sinks of carbon dioxide on Earth. While carbon sequestration in terrestrial plants is linked to the microorganisms living in their soils, the interactions of seagrasses with their rhizospheres are poorly understood. Here, we show that the seagrass, Posidonia oceanica excretes sugars, mainly sucrose, into its rhizosphere. These sugars accumulate to µM concentrations—nearly 80 times higher than previously observed in marine environments. This finding is unexpected as sugars are readily consumed by microorganisms. Our experiments indicated that under low oxygen conditions, phenolic compounds from P. oceanica inhibited microbial consumption of sucrose. Analyses of the rhizosphere community revealed that many microbes had the genes for degrading sucrose but these were only expressed by a few taxa that also expressed genes for degrading phenolics. Given that we observed high sucrose concentrations underneath three other species of marine plants, we predict that the presence of plant-produced phenolics under low oxygen conditions allows the accumulation of labile molecules across aquatic rhizospheres.

m/z_327.0165

Formula: - (n/a)
Adducts: (Ppm: 0)
Posidonia oceanica (root)
20190613_MS1_A19r-18
Resolution: 17μm, 246x264

Description

m/z_327.0163

Formula: - (n/a)
Adducts: (Ppm: 0)
Posidonia oceanica (root)
MS1_20180404_PO_1200
Resolution: 17μm, 193x208

Description

methoxychlor

Formula: C16H15Cl3O2 (344.0138)
Adducts: [M+H-H2O]+ (Ppm: 6.9)
Mus musculus (Liver)
Salmonella_final_pos_recal
Resolution: 17μm, 691x430

Description

A more complete and holistic view on host–microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium. [dataset] Nicole Strittmatter. Holistic Characterization of a Salmonella Typhimurium Infection Model Using Integrated Molecular Imaging, metabolights_dataset, V1; 2022. https://www.ebi.ac.uk/metabolights/MTBLS2671.