在BioDeep NovoCell知识数据库中,参考离子总共被划分为4个级别。
  • Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
  • Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
  • Unreliable: 这个参考离子具有较高的排名价值,但缺乏可重复性。
  • Unavailable: 由于排名价值低且缺乏可重复性,这个参考离子不应用于注释。

Found 8 Reference Ions Near m/z 251.0004
NovoCell ID m/z Mass Window Metabolite Ranking Anatomy Context
MSI_000054082 Unreliable 250.9916 250.9916 ~ 250.9918
MzDiff: 1.0 ppm
Thiarubrin A (BioDeep_00000006792)
Formula: C13H8S2 (228.0067)
3.46 (100%) MALDI - CHCA
[NOVOCELL:BACKGROUND] blank
MSI_000063348 Unreliable 250.9905 250.9905 ~ 250.9905
MzDiff: none
4-Chloro-5-sulfamoylanthranilic acid (BioDeep_00000054240)
Formula: C7H7ClN2O4S (249.9815)
0.23 (100%) Mus musculus
[UBERON:0000956] cerebral cortex
MSI_000060244 Unreliable 250.9905 250.9905 ~ 250.9905
MzDiff: 0.0 ppm
4-Chloro-5-sulfamoylanthranilic acid (BioDeep_00000054240)
Formula: C7H7ClN2O4S (249.9815)
0.14 (100%) Mus musculus
[UBERON:0002421] hippocampal formation
MSI_000036549 Unreliable 251.0001 251.0001 ~ 251.0001
MzDiff: none
Thienodolin (BioDeep_00000007256)
Formula: C11H7ClN2OS (249.9968)
2.04 (100%) Posidonia oceanica
[PO:0006036] root epidermis
MSI_000039865 Unreliable 251.0001 251.0001 ~ 251.0001
MzDiff: none
Thienodolin (BioDeep_00000007256)
Formula: C11H7ClN2OS (249.9968)
0.81 (100%) Posidonia oceanica
[PO:0005417] phloem
MSI_000039937 Unreliable 251.0104 251.0104 ~ 251.0104
MzDiff: none
4-chloro-9-methoxyfuro[3,2-g]chromen-7-one (BioDeep_00002063850)
Formula: C12H7ClO4 (250.0033)
0.41 (100%) Posidonia oceanica
[PO:0005417] phloem
MSI_000059158 Unreliable 250.9905 250.9905 ~ 250.9905
MzDiff: none
4-Chloro-5-sulfamoylanthranilic acid (BioDeep_00000054240)
Formula: C7H7ClN2O4S (249.9815)
1.58 (100%) Mus musculus
[UBERON:0001950] neocortex
MSI_000059755 Unavailable 250.991 250.991 ~ 250.991
MzDiff: none
4-Chloro-5-sulfamoylanthranilic acid (BioDeep_00000054240)
Formula: C7H7ClN2O4S (249.9815)
-0.37 (100%) Mus musculus
[UBERON:0002298] brainstem

Found 9 Sample Hits
Metabolite Species Sample
Dichlorophen

Formula: C13H10Cl2O2 (268.0058)
Adducts: [M+H-H2O]+ (Ppm: 8.3)
Marker Pen (NA)
3ul_0.8Mpa_RAW_20241016-PAPER PNMK
Resolution: 30μm, 315x42

Description

By writing the four English letters “PNMK” on white paper with a marker pen, and then scanning with a DESI ion source to obtain the scanning result. The signal of the chemical substances on the marker pen used appears on the channel with an m/z value of 322.1918, 323.1953, 546.4010, and etc, from the single cell deconvolution sampling layer class_4. This test data was tested by chuxiaoping from PANOMIX’s R&D laboratory.

Thienodolin

Formula: C11H7ClN2OS (249.9968)
Adducts: [M+H]+ (Ppm: 15.7)
Posidonia oceanica (root)
20190822_MS1_A19r-19
Resolution: 17μm, 303x309

Description

Seagrasses are among the most efficient sinks of carbon dioxide on Earth. While carbon sequestration in terrestrial plants is linked to the microorganisms living in their soils, the interactions of seagrasses with their rhizospheres are poorly understood. Here, we show that the seagrass, Posidonia oceanica excretes sugars, mainly sucrose, into its rhizosphere. These sugars accumulate to µM concentrations—nearly 80 times higher than previously observed in marine environments. This finding is unexpected as sugars are readily consumed by microorganisms. Our experiments indicated that under low oxygen conditions, phenolic compounds from P. oceanica inhibited microbial consumption of sucrose. Analyses of the rhizosphere community revealed that many microbes had the genes for degrading sucrose but these were only expressed by a few taxa that also expressed genes for degrading phenolics. Given that we observed high sucrose concentrations underneath three other species of marine plants, we predict that the presence of plant-produced phenolics under low oxygen conditions allows the accumulation of labile molecules across aquatic rhizospheres.

Thiarubrin A

Formula: C13H8S2 (228.0067)
Adducts: [M+Na]+ (Ppm: 15.7)
Posidonia oceanica (root)
20190828_MS1_A19r-22
Resolution: 17μm, 292x279

Description

Thienodolin

Formula: C11H7ClN2OS (249.9968)
Adducts: [M+H]+ (Ppm: 15.7)
Posidonia oceanica (root)
MS1_20180404_PO_1200
Resolution: 17μm, 193x208

Description

1-(5-Phenyl-1,2,4-thiadiazol-3-yl)-3-sulfanylideneurea

Formula: C9H6N4OS2 (249.9983)
Adducts: [M+H]+ (Ppm: 10.8)
Mus musculus (Liver)
Salmonella_final_pos_recal
Resolution: 17μm, 691x430

Description

A more complete and holistic view on host–microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium. [dataset] Nicole Strittmatter. Holistic Characterization of a Salmonella Typhimurium Infection Model Using Integrated Molecular Imaging, metabolights_dataset, V1; 2022. https://www.ebi.ac.uk/metabolights/MTBLS2671.

4-Chloro-5-sulfamoylanthranilic acid

Formula: C7H7ClN2O4S (249.9815)
Adducts: [M+H]+ (Ppm: 8.8)
Mus musculus (brain)
Brain01_Bregma-3-88b_centroid
Resolution: 17μm, 265x320

Description

4-Chloro-5-sulfamoylanthranilic acid

Formula: C7H7ClN2O4S (249.9815)
Adducts: [M+H]+ (Ppm: 6.8)
Mus musculus (brain)
Brain02_Bregma1-42_03
Resolution: 17μm, 483x403

Description

4-Chloro-5-sulfamoylanthranilic acid

Formula: C7H7ClN2O4S (249.9815)
Adducts: [M+H]+ (Ppm: 6.8)
Mus musculus (brain)
Brain02_Bregma-3-88
Resolution: 17μm, 288x282

Description

4-Chloro-5-sulfamoylanthranilic acid

Formula: C7H7ClN2O4S (249.9815)
Adducts: [M+H]+ (Ppm: 6.8)
Mus musculus (brain)
Brain02_Bregma-1-46
Resolution: 17μm, 294x399

Description