M/Z: 943.5873
Hit 4 annotations: PKG Inhibitor_[M+H]+; PS(22:4(7Z,10Z,13Z,16Z)/6 keto-PGF1alpha)_[M+NH4]+; PC(18:1(11Z)/LTE4)_[M+H]+; PI(22:3(10Z,13Z,16Z)/18:1(12Z)-O(9S,10R))_[M+H]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 4 Reference Ions Near m/z 943.5873
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000026940 Unreliable | 943.5815 | 943.5815 ~ 943.5815 MzDiff: none |
PC(18:1(11Z)/LTE4) (BioDeep_00000211341) Formula: C49H87N2O11PS (942.5768) |
1.78 (100%) | Mus musculus [UBERON:0002048] lung |
| MSI_000027032 Unreliable | 943.5932 | 943.5932 ~ 943.5932 MzDiff: none |
Alliosterol 1-(4'-galactosylrhamnoside) 16-galactoside (BioDeep_00000020816) Formula: C45H76O18 (904.5031) |
1.78 (100%) | Mus musculus [UBERON:0002048] lung |
| MSI_000049966 Unreliable | 943.5881 | 943.5881 ~ 943.5881 MzDiff: none |
PKG Inhibitor (BioDeep_00000182497) Formula: C38H74N18O10 (942.5835) |
1.3 (100%) | Mytilus edulis [UBERON:0009120] gill filament |
| MSI_000050972 Unreliable | 943.5881 | 943.5881 ~ 943.5881 MzDiff: none |
PKG Inhibitor (BioDeep_00000182497) Formula: C38H74N18O10 (942.5835) |
0.45 (100%) | Mytilus edulis [UBERON:2001856] gill ray |
Found 8 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| PKG Inhibitor Formula: C38H74N18O10 (942.5835) Adducts: [M+H]+ (Ppm: 1.7) |
Homo sapiens (Liver) |
20171107_FIT4_DHBpos_p70_s50Resolution: 50μm, 70x70
|
|
| PS(22:4(7Z,10Z,13Z,16Z)/6 keto-PGF1alpha) Formula: C48H80NO14P (925.5316) Adducts: [M+NH4]+ (Ppm: 15.6) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
| PC(18:1(11Z)/LTE4) Formula: C49H87N2O11PS (942.5768) Adducts: [M+H]+ (Ppm: 3.4) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
| PC(18:1(11Z)/LTE4) Formula: C49H87N2O11PS (942.5768) Adducts: [M+H]+ (Ppm: 1.2) |
Mus musculus (Lung) |
image3Resolution: 40μm, 146x190
Fig. 4 MALDI-MSI data of mouse lung tissue after administration with D9-choline and U13C-DPPC–containing Poractant alfa surfactant (labels administered 12 h prior to tissue collection). Ion images of (A) m/z 796.6856 ([U13C-DPPC+Na]+), (B) m/z 756.5154 [PC32:0+Na]+), and (C) m/z 765.6079 ([D9-PC32:0+Na]+). D: Overlay image of [U13C-PC32:0+Na]+ (red) and [D9-PC32:0+Na]+ (green). Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC. |
|
| PI(22:3(10Z,13Z,16Z)/18:1(12Z)-O(9S,10R)) Formula: C50H87O14P (942.5833) Adducts: [M+H]+ (Ppm: 3.3) |
Mus musculus (Lung) |
image5Resolution: 40μm, 163x183
Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and
U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion
images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079
([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green).
Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. |
|
| PKG Inhibitor Formula: C38H74N18O10 (942.5835) Adducts: [M+H]+ (Ppm: 1.9) |
Mytilus edulis (mantle) |
20190201_MS38_Crassostrea_Mantle_350-1500_DHB_pos_A28_10um_270x210Resolution: 10μm, 270x210
|
|
| PKG Inhibitor Formula: C38H74N18O10 (942.5835) Adducts: [M+H]+ (Ppm: 2.8) |
Mytilus edulis (gill) |
20190202_MS38_Crassostrea_Gill_350-1500_DHB_pos_A25_11um_305x210Resolution: 11μm, 305x210
single cell layer |
|
| PKG Inhibitor Formula: C38H74N18O10 (942.5835) Adducts: [M+H]+ (Ppm: 1.9) |
Mytilus edulis (mantle) |
20190216_MS38_Mytilus_mantle_350-1500_DHB_pos_A26_10um_275x210Resolution: 10μm, 275x210
|
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