M/Z: 925.7514

Hit 2 annotations: PC(22:1(13Z)/24:1(15Z))_[M]+; PE-NMe2(22:4(7Z,10Z,13Z,16Z)/24:0)_[M+NH4]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 3 Reference Ions Near m/z 925.7514

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000001326 Unavailable	925.7514	925.7514 ~ 925.7514 MzDiff: `none`	PC(22:1(13Z)/24:1(15Z)) (BioDeep_00000029842) Formula: C54H104NO8P (925.7499)	-0.58 (100%)	Mus musculus [UBERON:0001224] renal pelvis
MSI_000001771 Unavailable	925.7514	925.7514 ~ 925.7514 MzDiff: `none`	PC(22:1(13Z)/24:1(15Z)) (BioDeep_00000029842) Formula: C54H104NO8P (925.7499)	-0.58 (100%)	Mus musculus [UBERON:0001225] cortex of kidney
MSI_000002240 Unavailable	925.7514	925.7514 ~ 925.7514 MzDiff: `none`	PC(22:1(13Z)/24:1(15Z)) (BioDeep_00000029842) Formula: C54H104NO8P (925.7499)	-0.59 (100%)	Mus musculus [UBERON:0001293] outer medulla of kidney

Found 3 Sample Hits

Metabolite	Species	Sample
PC(22:1(13Z)/24:1(15Z)) Formula: C54H104NO8P (925.7499) Adducts: [M]+ (Ppm: 2.2)	Mus musculus (Kidney)	FULL_MS_centriod_CHCA_20210819 Resolution: 17μm, 638x437 Description AP-MALDI instrument demo test, mass spectrum scan in centroid mode.
m/z_925.7556 Formula: - (n/a) Adducts: (Ppm: )	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.
PE-NMe2(22:4(7Z,10Z,13Z,16Z)/24:0) Formula: C53H98NO8P (907.703) Adducts: [M+NH4]+ (Ppm: 15.5)	Mus musculus (Lung)	image2 Resolution: 40μm, 550x256 Description Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b) [PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9- choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using total ion current normalisation and hotspot removal (high quantile = 99%).

Metabolite

Species

Sample

PC(22:1(13Z)/24:1(15Z))

Formula: C54H104NO8P (925.7499)
Adducts: [M]+ (Ppm: 2.2)

Mus musculus (Kidney)

FULL_MS_centriod_CHCA_20210819

Resolution: 17μm, 638x437

Description

AP-MALDI instrument demo test, mass spectrum scan in centroid mode.

m/z_925.7556

Formula: - (n/a)
Adducts: (Ppm: )

Mus musculus (Lung)

image5

Resolution: 40μm, 163x183

Description

Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.

PE-NMe2(22:4(7Z,10Z,13Z,16Z)/24:0)

Formula: C53H98NO8P (907.703)
Adducts: [M+NH4]+ (Ppm: 15.5)

Mus musculus (Lung)

image2

Resolution: 40μm, 550x256

Description

Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b) [PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9- choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using total ion current normalisation and hotspot removal (high quantile = 99%).