M/Z: 854.639
Hit 3 annotations: PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z))_[M+H-2H2O]+; LacCer(d18:1/18:0)_[M+H-2H2O]+; PC(20:0/20:3(5Z,8Z,11Z)-O(14R,15S))_[M+H]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 12 Reference Ions Near m/z 854.639
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000002863 Unavailable | 854.639 | 854.639 ~ 854.639 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
-0.49 (100%) | Rattus norvegicus [UBERON:0001950] neocortex |
| MSI_000003376 Unavailable | 854.639 | 854.639 ~ 854.639 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
-0.31 (100%) | Rattus norvegicus [UBERON:0002037] cerebellum |
| MSI_000005018 Unavailable | 854.639 | 854.639 ~ 854.639 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
-0.4 (100%) | Rattus norvegicus [UBERON:0002298] brainstem |
| MSI_000005394 Unreliable | 854.639 | 854.639 ~ 854.639 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
2.46 (100%) | Rattus norvegicus [UBERON:0002435] striatum |
| MSI_000061768 Unavailable | 854.6395 | 854.6395 ~ 854.6395 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
-0.39 (100%) | Mus musculus [UBERON:0000956] cerebral cortex |
| MSI_000062501 Unavailable | 854.6395 | 854.6395 ~ 854.6395 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
-0.39 (100%) | Mus musculus [UBERON:0001950] neocortex |
| MSI_000062553 Unreliable | 854.6395 | 854.6395 ~ 854.6395 MzDiff: none |
LacCer(d18:1/18:0) (BioDeep_00000032128) Formula: C48H91NO13 (889.649) |
2.47 (100%) | Mus musculus [UBERON:0002421] hippocampal formation |
| MSI_000001389 Unavailable | 854.6445 | 854.6445 ~ 854.6445 MzDiff: none |
PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z)) (BioDeep_00000029777) Formula: C52H92NO8P (889.656) |
-0.65 (100%) | Mus musculus [UBERON:0001224] renal pelvis |
| MSI_000001594 Unavailable | 854.6445 | 854.6445 ~ 854.6445 MzDiff: none |
PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z)) (BioDeep_00000029777) Formula: C52H92NO8P (889.656) |
-0.41 (100%) | Mus musculus [UBERON:0001225] cortex of kidney |
| MSI_000002220 Unavailable | 854.6445 | 854.6445 ~ 854.6445 MzDiff: none |
PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z)) (BioDeep_00000029777) Formula: C52H92NO8P (889.656) |
-0.56 (100%) | Mus musculus [UBERON:0001293] outer medulla of kidney |
| MSI_000050103 Unreliable | 854.6458 | 854.6458 ~ 854.6458 MzDiff: none |
PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z)) (BioDeep_00000029777) Formula: C52H92NO8P (889.656) |
0.91 (100%) | Mytilus edulis [UBERON:0009120] gill filament |
| MSI_000050580 Unreliable | 854.6458 | 854.6458 ~ 854.6458 MzDiff: none |
PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z)) (BioDeep_00000029777) Formula: C52H92NO8P (889.656) |
1.82 (100%) | Mytilus edulis [UBERON:2001856] gill ray |
Found 4 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| PC(20:5(5Z,8Z,11Z,14Z,17Z)/24:1(15Z)) Formula: C52H92NO8P (889.656) Adducts: [M+H-2H2O]+ (Ppm: 2.7) |
Mus musculus (Kidney) |
FULL_MS_centriod_CHCA_20210819Resolution: 17μm, 638x437
AP-MALDI instrument demo test, mass spectrum scan in centroid mode. |
|
| LacCer(d18:1/18:0) Formula: C48H91NO13 (889.649) Adducts: [M+H-2H2O]+ (Ppm: 4.5) |
Rattus norvegicus (Brain) |
Spectroswiss - sol_2x_br_2Resolution: 17μm, 488x193
|
|
| PC(20:0/20:3(5Z,8Z,11Z)-O(14R,15S)) Formula: C48H88NO9P (853.6196) Adducts: [M+H]+ (Ppm: 19.1) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
| LacCer(d18:1/18:0) Formula: C48H91NO13 (889.649) Adducts: [M+H-2H2O]+ (Ppm: 5.1) |
Mus musculus (brain) |
Brain01_Bregma1-42_02_centroidResolution: 17μm, 434x258
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