M/Z: 832.635

Hit 2 annotations: PA(22:5(4Z,7Z,10Z,13Z,16Z)/24:1(15Z))_[M-H2O+NH4]+; PA(a-25:0/18:1(12Z)-O(9S,10R))_[M+NH4]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 2 Reference Ions Near m/z 832.635

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000047423 Unreliable	832.6255	832.6255 ~ 832.6255 MzDiff: `none`	PA(22:5(4Z,7Z,10Z,13Z,16Z)/24:1(15Z)) (BioDeep_00000107731) Formula: C49H85O8P (832.5982)	2.3 (100%)	Homo sapiens [UBERON:0013067] colorectal mucosa
MSI_000052517 Unreliable	832.6292	832.6292 ~ 832.6292 MzDiff: `none`	Not Annotated	1.85 (0%)	Homo sapiens [UBERON:0001052] rectum

Found 2 Sample Hits

Metabolite	Species	Sample
PA(22:5(4Z,7Z,10Z,13Z,16Z)/24:1(15Z)) Formula: C49H85O8P (832.5982) Adducts: [M-H2O+NH4]+ (Ppm: 16.3)	Mus musculus (Lung)	image1 Resolution: 40μm, 187x165 Description Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5
PA(a-25:0/18:1(12Z)-O(9S,10R)) Formula: C46H87O9P (814.6087) Adducts: [M+NH4]+ (Ppm: 14.5)	Homo sapiens (colorectal adenocarcinoma)	520TopL, 490TopR, 510BottomL, 500BottomR-profile Resolution: 17μm, 147x131 Description The human colorectal adenocarcinoma sample was excised during a surgical operation performed at the Imperial College Healthcare NHS Trust. The sample and procedures were carried out in accordance with ethical approval (14/EE/0024).

Metabolite

Species

Sample

PA(22:5(4Z,7Z,10Z,13Z,16Z)/24:1(15Z))

Formula: C49H85O8P (832.5982)
Adducts: [M-H2O+NH4]+ (Ppm: 16.3)

Mus musculus (Lung)

image1

Resolution: 40μm, 187x165

Description

Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5

PA(a-25:0/18:1(12Z)-O(9S,10R))

Formula: C46H87O9P (814.6087)
Adducts: [M+NH4]+ (Ppm: 14.5)

Homo sapiens (colorectal adenocarcinoma)

520TopL, 490TopR, 510BottomL, 500BottomR-profile

Resolution: 17μm, 147x131

Description

The human colorectal adenocarcinoma sample was excised during a surgical operation performed at the Imperial College Healthcare NHS Trust. The sample and procedures were carried out in accordance with ethical approval (14/EE/0024).