M/Z: 749.5692
Hit 2 annotations: DG(21:0/22:6(5Z,7Z,10Z,13Z,16Z,19Z)-OH(4)/0:0)_[M+Na]+; Cer(d20:1/LTE4)_[M+H]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 5 Reference Ions Near m/z 749.5692
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000025391 Unreliable | 749.5785 | 749.5784 ~ 749.5788 MzDiff: 1.6 ppm |
Not Annotated | 3.62 (0%) | Mus musculus [UBERON:0000913] interstitial fluid |
| MSI_000053096 Unreliable | 749.5596 | 749.5596 ~ 749.5596 MzDiff: none |
Not Annotated | 2.97 (0%) | DESI [NOVOCELL:BACKGROUND] blank |
| MSI_000027008 Unreliable | 749.5692 | 749.5692 ~ 749.5692 MzDiff: none |
Not Annotated | 1.78 (0%) | Mus musculus [UBERON:0002048] lung |
| MSI_000027112 Unreliable | 749.5607 | 749.5607 ~ 749.5607 MzDiff: none |
Cer(d20:1/LTE4) (BioDeep_00000215110) Formula: C43H76N2O6S (748.5424) |
1.77 (100%) | Mus musculus [UBERON:0002048] lung |
| MSI_000027772 Unreliable | 749.5662 | 749.5662 ~ 749.5662 MzDiff: none |
PE(P-16:0/22:5(4Z,7Z,10Z,13Z,16Z)) (BioDeep_00000018547) Formula: C43H76NO7P (749.5359) |
2.04 (100%) | Mus musculus [UBERON:0002048] lung |
Found 3 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| DG(21:0/22:6(5Z,7Z,10Z,13Z,16Z,19Z)-OH(4)/0:0) Formula: C46H78O6 (726.5798) Adducts: [M+Na]+ (Ppm: 3.8) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
| Cer(d20:1/LTE4) Formula: C43H76N2O6S (748.5424) Adducts: [M+H]+ (Ppm: 14.7) |
Mus musculus (Lung) |
image4Resolution: 40μm, 162x156
Fig 6c
Fig. 6 MALDI-MSI of U13C-PC16:0/16:0 acyl chain remodeling. A: Averaged MALDI mass spectrum from lung tissue collected from mice euthanized 12 h after administration of D9-choline and U13C-DPPC–containing Poractant alfa surfactant. The ion at m/z 828.6321 is assigned as the [M+Na]+ ion of 13C24-PC16:0_20:4 formed by acyl remodeling of U13C-PC16:0/16:0. The “NL” value refers to the intensity of the base peak in the full range MS1 spectrum. B: MS/MS spectrum of precursor ions at m/z 828.5 ± 0.5 with fragment ions originating from [13C24-PC16:0_20:4+Na]+ annotated. Part-per-million (ppm) mass errors are provided in parentheses. C, D: MALDI-MSI data of [U13C-DPPC+Na]+ (blue), [PC36:4+Na]+ (green) and [13C24-PC16:0_20:4+Na]+ (red) in lung tissue collected from mice (C) 12 h and (D) 18 h after label administration. All images were visualized using total-ion-current normalization and hotspot removal (high quantile = 99%). MS/MS, tandem mass spectrometry; MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC. |
|
| m/z_749.5692 Formula: - (n/a) Adducts: (Ppm: 0) |
Mus musculus (Lung) |
image4Resolution: 40μm, 162x156
Fig 6c
Fig. 6 MALDI-MSI of U13C-PC16:0/16:0 acyl chain remodeling. A: Averaged MALDI mass spectrum from lung tissue collected from mice euthanized 12 h after administration of D9-choline and U13C-DPPC–containing Poractant alfa surfactant. The ion at m/z 828.6321 is assigned as the [M+Na]+ ion of 13C24-PC16:0_20:4 formed by acyl remodeling of U13C-PC16:0/16:0. The “NL” value refers to the intensity of the base peak in the full range MS1 spectrum. B: MS/MS spectrum of precursor ions at m/z 828.5 ± 0.5 with fragment ions originating from [13C24-PC16:0_20:4+Na]+ annotated. Part-per-million (ppm) mass errors are provided in parentheses. C, D: MALDI-MSI data of [U13C-DPPC+Na]+ (blue), [PC36:4+Na]+ (green) and [13C24-PC16:0_20:4+Na]+ (red) in lung tissue collected from mice (C) 12 h and (D) 18 h after label administration. All images were visualized using total-ion-current normalization and hotspot removal (high quantile = 99%). MS/MS, tandem mass spectrometry; MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC. |
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