M/Z: 743.4623
Hit 2 annotations: Alliosterol 1-rhamnoside 16-galactoside_[M+H]+
; PA(20:5(5Z,8Z,11Z,14Z,17Z)/20:4(8Z,11Z,14Z,17Z))_[M+H]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值,但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性,这个参考离子不应用于注释。
Found 2 Reference Ions Near m/z 743.4623
NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
---|---|---|---|---|---|
MSI_000049965 Unreliable | 743.4621 | 743.4621 ~ 743.4621 MzDiff: none |
Alliosterol 1-rhamnoside 16-galactoside (BioDeep_00000021219) Formula: C39H66O13 (742.4503) |
1.3 (100%) | Mytilus edulis [UBERON:0009120] gill filament |
MSI_000051031 Unreliable | 743.4621 | 743.4621 ~ 743.4621 MzDiff: none |
Alliosterol 1-rhamnoside 16-galactoside (BioDeep_00000021219) Formula: C39H66O13 (742.4503) |
0.26 (100%) | Mytilus edulis [UBERON:2001856] gill ray |
Found 7 Sample Hits
Metabolite | Species | Sample | |
---|---|---|---|
Alliosterol 1-rhamnoside 16-galactoside Formula: C39H66O13 (742.4503) Adducts: [M+H]+ (Ppm: 5.1) |
Homo sapiens (Liver) |
20171107_FIT4_DHBpos_p70_s50Resolution: 50μm, 70x70
|
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Alliosterol 1-rhamnoside 16-galactoside Formula: C39H66O13 (742.4503) Adducts: [M+H]+ (Ppm: 7) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
PA(20:5(5Z,8Z,11Z,14Z,17Z)/20:4(8Z,11Z,14Z,17Z)) Formula: C43H67O8P (742.4573) Adducts: [M+H]+ (Ppm: 2.4) |
Mytilus edulis (mantle) |
20190201_MS38_Crassostrea_Mantle_350-1500_DHB_pos_A28_10um_270x210Resolution: 10μm, 270x210
|
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Alliosterol 1-rhamnoside 16-galactoside Formula: C39H66O13 (742.4503) Adducts: [M+H]+ (Ppm: 6.1) |
Mytilus edulis (gill) |
20190202_MS38_Crassostrea_Gill_350-1500_DHB_pos_A25_11um_305x210Resolution: 11μm, 305x210
single cell layer |
|
Alliosterol 1-rhamnoside 16-galactoside Formula: C39H66O13 (742.4503) Adducts: [M+H]+ (Ppm: 6.3) |
Mytilus edulis (mantle) |
20190216_MS38_Mytilus_mantle_350-1500_DHB_pos_A26_10um_275x210Resolution: 10μm, 275x210
|
|
PA(20:5(5Z,8Z,11Z,14Z,17Z)/20:4(8Z,11Z,14Z,17Z)) Formula: C43H67O8P (742.4573) Adducts: [M+H]+ (Ppm: 0.5) |
Mus musculus (brain) |
Brain02_Bregma-3-88Resolution: 17μm, 288x282
|
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Alliosterol 1-rhamnoside 16-galactoside Formula: C39H66O13 (742.4503) Adducts: [M+H]+ (Ppm: 4.8) |
Drosophila melanogaster (brain) |
Drosophila18Resolution: 5μm, 686x685
Sample information
Organism: Drosophila melanogaster
Organism part: Brain
Condition: Healthy
Sample preparation
Sample stabilisation: Frozen
Tissue modification: Frozen
MALDI matrix: 2,5-dihydroxybenzoic acid (DHB)
MALDI matrix application: TM sprayer
Solvent: Aceton/water
MS analysis
Polarity: Positive
Ionisation source: Prototype
Analyzer: Orbitrap
Pixel size: 5μm × 5μm
Annotation settings
m/z tolerance (ppm): 3
Analysis version: Original MSM
Pixel count: 469910
Imzml file size: 696.23 MB
Ibd file size: 814.11 MB |
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