M/Z: 717.5623

Hit 2 annotations: PE(16:0/18:1(9Z))_[M-H2O+NH4]+; DG(21:0/PGJ2/0:0)_[M+H]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 1 Reference Ions Near m/z 717.5623

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000010005 Unreliable	717.5623	717.5623 ~ 717.5623 MzDiff: `none`	PE(16:0/18:1(9Z)) (BioDeep_00000004081) Formula: C39H76NO8P (717.5308)	1.17 (100%)	Bathymodiolus [UBERON:0009120] gill filament

Found 3 Sample Hits

Metabolite	Species	Sample
PE(16:0/18:1(9Z)) Formula: C39H76NO8P (717.5308) Adducts: [M-H2O+NH4]+ (Ppm: 11.4)	Bathymodiolus (epithelial host cells)	MPIMM_039_QE_P_BP_CF_Bputeoserpentis_MALDI-FISH8_Sl14_s1_DHB_233x233_3um Resolution: 3μm, 233x234 Description
DG(21:0/PGJ2/0:0) Formula: C44H76O7 (716.5591) Adducts: [M+H]+ (Ppm: 10.2)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.
PE(16:0/18:1(9Z)) Formula: C39H76NO8P (717.5308) Adducts: [M-H2O+NH4]+ (Ppm: 4.3)	Mus musculus (Lung)	image2 Resolution: 40μm, 550x256 Description Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b) [PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9- choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using total ion current normalisation and hotspot removal (high quantile = 99%).

Metabolite

Species

Sample

PE(16:0/18:1(9Z))

Formula: C39H76NO8P (717.5308)
Adducts: [M-H2O+NH4]+ (Ppm: 11.4)

Bathymodiolus (epithelial host cells)

MPIMM_039_QE_P_BP_CF_Bputeoserpentis_MALDI-FISH8_Sl14_s1_DHB_233x233_3um

Resolution: 3μm, 233x234

Description

DG(21:0/PGJ2/0:0)

Formula: C44H76O7 (716.5591)
Adducts: [M+H]+ (Ppm: 10.2)

Mus musculus (Lung)

image5

Resolution: 40μm, 163x183

Description

Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.

PE(16:0/18:1(9Z))

Formula: C39H76NO8P (717.5308)
Adducts: [M-H2O+NH4]+ (Ppm: 4.3)

Mus musculus (Lung)

image2

Resolution: 40μm, 550x256

Description

Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b) [PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9- choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using total ion current normalisation and hotspot removal (high quantile = 99%).