M/Z: 668.4487
Hit 3 annotations: Pepstatin_[M+H-H2O]+; PA(14:0/20:4(5Z,8Z,11Z,14Z))_[M]+; PA(12:0/18:1(12Z)-2OH(9,10))_[M+NH4]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 3 Reference Ions Near m/z 668.4487
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000023290 Unreliable | 668.4487 | 668.4487 ~ 668.4487 MzDiff: none |
Pepstatin (BioDeep_00000182236) Formula: C34H63N5O9 (685.4626) |
1.78 (100%) | Mus musculus [UBERON:0004263] upper arm skin |
| MSI_000023677 Unreliable | 668.4404 | 668.4404 ~ 668.4404 MzDiff: none |
PA(12:0/18:1(12Z)-2OH(9,10)) (BioDeep_00000187796) Formula: C33H63O10P (650.4159) |
1.29 (100%) | Mus musculus [UBERON:0004263] upper arm skin |
| MSI_000044910 Unavailable | 668.44 | 668.44 ~ 668.44 MzDiff: none |
Arjunolic acid 3-glucoside (BioDeep_00000019048) Formula: C36H58O10 (650.403) |
-0.52 (100%) | Rattus norvegicus [UBERON:0002264] olfactory bulb |
Found 5 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| Pepstatin Formula: C34H63N5O9 (685.4626) Adducts: [M+H-H2O]+ (Ppm: 15.8) |
Mus musculus (Left upper arm) |
357_l_total ion countResolution: 50μm, 97x131
Diseased |
|
| PA(14:0/20:4(5Z,8Z,11Z,14Z)) Formula: C37H65O8P (668.4417) Adducts: [M]+ (Ppm: 6.5) |
Mus musculus (Lung) |
image5Resolution: 40μm, 163x183
Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and
U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion
images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079
([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green).
Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. |
|
| m/z_668.4432 Formula: - (n/a) Adducts: (Ppm: 0) |
Mus musculus (Lung) |
image2Resolution: 40μm, 550x256
Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b)
[PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9-
choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in
parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in
white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar
regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids
PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using
total ion current normalisation and hotspot removal (high quantile = 99%). |
|
| Pepstatin Formula: C34H63N5O9 (685.4626) Adducts: [M+H-H2O]+ (Ppm: 12.4) |
Mus musculus (Lung) |
image2Resolution: 40μm, 550x256
Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b)
[PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9-
choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in
parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in
white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar
regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids
PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using
total ion current normalisation and hotspot removal (high quantile = 99%). |
|
| PA(12:0/18:1(12Z)-2OH(9,10)) Formula: C33H63O10P (650.4159) Adducts: [M+NH4]+ (Ppm: 4.8) |
Homo sapiens (esophagus) |
LNTO29_16_2Resolution: 17μm, 95x101
|
|
