M/Z: 659.0321

Hit 2 annotations: Lonafarnib_[M+Na]+; sarasar_[M+Na]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 5 Reference Ions Near m/z 659.0321

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000017857 Reliable	659.0365	659.0364 ~ 659.0365 MzDiff: `0.6 ppm`	sarasar (BioDeep_00002249075) Formula: C27H31Br2ClN4O2 (636.0502)	1.25 (100%)	Vitis vinifera [PO:0009087] mesocarp
MSI_000016882 Unavailable	659.0365	659.0364 ~ 659.0365 MzDiff: `0.6 ppm`	sarasar (BioDeep_00002249075) Formula: C27H31Br2ClN4O2 (636.0502)	-0.16 (100%)	Vitis vinifera [PO:0009086] endocarp
MSI_000055011 Unreliable	659.039	659.039 ~ 659.039 MzDiff: `none`	Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502)	1.57 (100%)	MALDI - DHB [NOVOCELL:BACKGROUND] blank
MSI_000004676 Unreliable	659.0321	659.0321 ~ 659.0321 MzDiff: `none`	Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502)	0.33 (100%)	Homo sapiens [UBERON:0002107] liver
MSI_000024733 Unreliable	659.0289	659.0289 ~ 659.0289 MzDiff: `none`	Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502)	1.77 (100%)	Mus musculus [UBERON:0004269] upper arm connective tissue

Found 7 Sample Hits

Metabolite	Species	Sample
Lonafarnib Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+Na]+ (Ppm: 11.1)	Homo sapiens (Liver)	20171107_FIT4_DHBpos_p70_s50 Resolution: 50μm, 70x70 Description
sarasar Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+Na]+ (Ppm: 4.5)	Vitis vinifera (Fruit)	grape_dhb_91_1 Resolution: 50μm, 120x114 Description Grape berries fruit, condition: Ripe
sarasar Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+Na]+ (Ppm: 4.6)	Vitis vinifera (Fruit)	grape_dhb_164_1 Resolution: 17μm, 136x122 Description Grape berries fruit, condition: Late
sarasar Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+Na]+ (Ppm: 4.6)	Vitis vinifera (Fruit)	grape_dhb_163_1 Resolution: 17μm, 132x115 Description Grape berries fruit, condition: Late
m/z_659.0302 Formula: - (n/a) Adducts: (Ppm: 0)	Mus musculus (Lung)	image4 Resolution: 40μm, 162x156 Description Fig 6c Fig. 6 MALDI-MSI of U13C-PC16:0/16:0 acyl chain remodeling. A: Averaged MALDI mass spectrum from lung tissue collected from mice euthanized 12 h after administration of D9-choline and U13C-DPPC–containing Poractant alfa surfactant. The ion at m/z 828.6321 is assigned as the [M+Na]+ ion of 13C24-PC16:0_20:4 formed by acyl remodeling of U13C-PC16:0/16:0. The “NL” value refers to the intensity of the base peak in the full range MS1 spectrum. B: MS/MS spectrum of precursor ions at m/z 828.5 ± 0.5 with fragment ions originating from [13C24-PC16:0_20:4+Na]+ annotated. Part-per-million (ppm) mass errors are provided in parentheses. C, D: MALDI-MSI data of [U13C-DPPC+Na]+ (blue), [PC36:4+Na]+ (green) and [13C24-PC16:0_20:4+Na]+ (red) in lung tissue collected from mice (C) 12 h and (D) 18 h after label administration. All images were visualized using total-ion-current normalization and hotspot removal (high quantile = 99%). MS/MS, tandem mass spectrometry; MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC.
m/z_659.0282 Formula: - (n/a) Adducts: (Ppm: 0)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.
Lonafarnib Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+Na]+ (Ppm: 3.4)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.