M/Z: 657.3853

Hit 1 annotations: (1s,3as,5ar,7r,8s,9ar,9br,11ar)-3a,8-dihydroxy-9a,11a-dimethyl-1-[(2s,3r,5r)-2,3,6-trihydroxy-5,6-dimethylheptan-2-yl]-7-{[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-1h,2h,3h,5ah,6h,7h,8h,9h,9bh,10h,11h-cyclopenta[a]phenanthren-5-one_[M+H]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 4 Reference Ions Near m/z 657.3853

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000021466 Unreliable	657.392	657.392 ~ 657.392 MzDiff: `none`	Not Annotated	0.9 (0%)	Mus musculus [UBERON:0001499] muscle of arm
MSI_000021978 Unreliable	657.3853	657.3853 ~ 657.3853 MzDiff: `none`	Not Annotated	0.51 (0%)	Mus musculus [UBERON:0001499] muscle of arm
MSI_000010007 Unreliable	657.3852	657.3852 ~ 657.3852 MzDiff: `none`	(1s,3as,5ar,7r,8s,9ar,9br,11ar)-3a,8-dihydroxy-9a,11a-dimethyl-1-[(2s,3r,5r)-2,3,6-trihydroxy-5,6-dimethylheptan-2-yl]-7-{[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-1h,2h,3h,5ah,6h,7h,8h,9h,9bh,10h,11h-cyclopenta[a]phenanthren-5-one (BioDeep_00002059684) Formula: C34H56O12 (656.3772)	1.14 (100%)	Bathymodiolus [UBERON:0009120] gill filament
MSI_000022859 Unreliable	657.3853	657.3853 ~ 657.3853 MzDiff: `none`	Not Annotated	0.93 (0%)	Mus musculus [UBERON:0004250] upper arm bone

Found 4 Sample Hits

Metabolite	Species	Sample
(1s,3as,5ar,7r,8s,9ar,9br,11ar)-3a,8-dihydroxy-9a,11a-dimethyl-1-[(2s,3r,5r)-2,3,6-trihydroxy-5,6-dimethylheptan-2-yl]-7-{[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-1h,2h,3h,5ah,6h,7h,8h,9h,9bh,10h,11h-cyclopenta[a]phenanthren-5-one Formula: C34H56O12 (656.3772) Adducts: [M+H]+ (Ppm: 1.2)	Bathymodiolus (epithelial host cells)	MPIMM_039_QE_P_BP_CF_Bputeoserpentis_MALDI-FISH8_Sl14_s1_DHB_233x233_3um Resolution: 3μm, 233x234 Description
m/z_657.3889 Formula: - (n/a) Adducts: (Ppm: 0)	Mus musculus (Lung)	image1 Resolution: 40μm, 187x165 Description Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5
m/z_657.3853 Formula: - (n/a) Adducts: (Ppm: 0)	Mus musculus (Left upper arm)	357_l_total ion count Resolution: 50μm, 97x131 Description Diseased
m/z_657.3902 Formula: - (n/a) Adducts: (Ppm: 0)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.