- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 6 Reference Ions Near m/z 654.1011
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000010363 Unavailable | 654.0951 | 654.0951 ~ 654.0952 MzDiff: 0.0 ppm |
Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502) |
-0.99 (100%) | Bathymodiolus [UBERON:0009120] gill filament |
| MSI_000012176 Unavailable | 654.0951 | 654.0951 ~ 654.0952 MzDiff: 0.0 ppm |
Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502) |
-0.98 (100%) | Bathymodiolus [UBERON:2000211] gill lamella |
| MSI_000054924 Unreliable | 654.0964 | 654.0964 ~ 654.0964 MzDiff: none |
Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502) |
1.64 (100%) | MALDI - DHB [NOVOCELL:BACKGROUND] blank |
| MSI_000004326 Unreliable | 654.0995 | 654.0995 ~ 654.0995 MzDiff: none |
Cytidylyl-(5'-3')-5'-inosinic acid homopolymer (BioDeep_00000182527) Formula: C19H25N7O15P2 (653.0884) |
0.76 (100%) | Homo sapiens [UBERON:0002107] liver |
| MSI_000010180 Unavailable | 654.0969 | 654.0969 ~ 654.0969 MzDiff: none |
Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502) |
-0.49 (100%) | Bathymodiolus [UBERON:0009120] gill filament |
| MSI_000027456 Unreliable | 654.0962 | 654.0962 ~ 654.0962 MzDiff: none |
Lonafarnib (BioDeep_00000229516) Formula: C27H31Br2ClN4O2 (636.0502) |
1.99 (100%) | Mus musculus [UBERON:0000913] interstitial fluid |
Found 8 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| Lonafarnib Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+NH4]+ (Ppm: 16.9) |
Bathymodiolus (epithelial host cells) |
MPIMM_054_QE_P_BP_CF_Bputeoserpentis_MALDI-FISH8_Sl16_s1_DHB_233x233_3umResolution: 3μm, 233x233
|
|
| Lonafarnib Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+NH4]+ (Ppm: 19.7) |
Bathymodiolus (epithelial host cells) |
MPIMM_039_QE_P_BP_CF_Bputeoserpentis_MALDI-FISH8_Sl14_s1_DHB_233x233_3umResolution: 3μm, 233x234
|
|
| Cytidylyl-(5'-3')-5'-inosinic acid homopolymer Formula: C19H25N7O15P2 (653.0884) Adducts: [M+H]+ (Ppm: 5.9) |
Homo sapiens (Liver) |
20171107_FIT4_DHBpos_p70_s50Resolution: 50μm, 70x70
|
|
| Cytidylyl-(5'-3')-5'-inosinic acid homopolymer Formula: C19H25N7O15P2 (653.0884) Adducts: [M+H]+ (Ppm: 4) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
| Lonafarnib Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+NH4]+ (Ppm: 18.6) |
Mus musculus (Lung) |
image2Resolution: 40μm, 550x256
Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b)
[PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9-
choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in
parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in
white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar
regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids
PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using
total ion current normalisation and hotspot removal (high quantile = 99%). |
|
| {8-[(2r,3s)-5,7-dihydroxy-2-(4-hydroxyphenyl)-4-oxo-2,3-dihydro-1-benzopyran-3-yl]-2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl}oxidanesulfonic acid Formula: C30H20O14S (636.0574) Adducts: [M+NH4]+ (Ppm: 15.1) |
Posidonia oceanica (root) |
20190613_MS1_A19r-18Resolution: 17μm, 246x264
|
|
| Lonafarnib Formula: C27H31Br2ClN4O2 (636.0502) Adducts: [M+NH4]+ (Ppm: 18.9) |
Mytilus edulis (mantle) |
20190201_MS38_Crassostrea_Mantle_350-1500_DHB_pos_A28_10um_270x210Resolution: 10μm, 270x210
|
|
| m/z_654.0985 Formula: - (n/a) Adducts: (Ppm: 0) |
Drosophila melanogaster (brain) |
Drosophila18Resolution: 5μm, 686x685
Sample information
Organism: Drosophila melanogaster
Organism part: Brain
Condition: Healthy
Sample preparation
Sample stabilisation: Frozen
Tissue modification: Frozen
MALDI matrix: 2,5-dihydroxybenzoic acid (DHB)
MALDI matrix application: TM sprayer
Solvent: Aceton/water
MS analysis
Polarity: Positive
Ionisation source: Prototype
Analyzer: Orbitrap
Pixel size: 5μm × 5μm
Annotation settings
m/z tolerance (ppm): 3
Analysis version: Original MSM
Pixel count: 469910
Imzml file size: 696.23 MB
Ibd file size: 814.11 MB |
|
