M/Z: 645.3225
Hit 2 annotations: Benzoyl-fvr-pna_[M+H]+; Canarigenin 3-[glucosyl-(1->4)-6-deoxy-alloside]_[M+H-2H2O]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。
Found 5 Reference Ions Near m/z 645.3225
| NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
|---|---|---|---|---|---|
| MSI_000058024 Reliable | 645.3268 | 645.3262 ~ 645.3269 MzDiff: 3.1 ppm |
Canarigenin 3-[glucosyl-(1->4)-6-deoxy-alloside] (BioDeep_00000034827) Formula: C35H52O13 (680.3408) |
5.83 (77%) | DESI [NOVOCELL:BACKGROUND] blank |
| MSI_000053002 Unreliable | 645.3213 | 645.3213 ~ 645.3213 MzDiff: none |
Benzoyl-fvr-pna (BioDeep_00000176577) Formula: C33H40N8O6 (644.3071) |
3.08 (100%) | DESI [NOVOCELL:BACKGROUND] blank |
| MSI_000052991 Unreliable | 645.3296 | 645.3296 ~ 645.3296 MzDiff: none |
Canarigenin 3-[glucosyl-(1->4)-6-deoxy-alloside] (BioDeep_00000034827) Formula: C35H52O13 (680.3408) |
3.09 (100%) | DESI [NOVOCELL:BACKGROUND] blank |
| MSI_000044618 Unavailable | 645.3164 | 645.3164 ~ 645.3164 MzDiff: none |
Benzoyl-fvr-pna (BioDeep_00000176577) Formula: C33H40N8O6 (644.3071) |
-0.39 (100%) | Rattus norvegicus [UBERON:0002264] olfactory bulb |
| MSI_000028031 Unreliable | 645.3169 | 645.3169 ~ 645.3169 MzDiff: none |
Benzoyl-fvr-pna (BioDeep_00000176577) Formula: C33H40N8O6 (644.3071) |
1.81 (100%) | Mus musculus [UBERON:0002048] lung |
Found 5 Sample Hits
| Metabolite | Species | Sample | |
|---|---|---|---|
| Benzoyl-fvr-pna Formula: C33H40N8O6 (644.3071) Adducts: [M+H]+ (Ppm: 6.9) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
| Benzoyl-fvr-pna Formula: C33H40N8O6 (644.3071) Adducts: [M+H]+ (Ppm: 10.8) |
Homo sapiens (colorectal adenocarcinoma) |
80TopL, 50TopR, 70BottomL, 60BottomR-profileResolution: 17μm, 137x136
The human colorectal adenocarcinoma sample was excised during a surgical operation performed at the Imperial College Healthcare NHS Trust. The sample and procedures were carried out in accordance with ethical approval (14/EE/0024). |
|
| Benzoyl-fvr-pna Formula: C33H40N8O6 (644.3071) Adducts: [M+H]+ (Ppm: 12.6) |
Homo sapiens (colorectal adenocarcinoma) |
520TopL, 490TopR, 510BottomL, 500BottomR-profileResolution: 17μm, 147x131
The human colorectal adenocarcinoma sample was excised during a surgical operation performed at the Imperial College Healthcare NHS Trust. The sample and procedures were carried out in accordance with ethical approval (14/EE/0024). |
|
| Canarigenin 3-[glucosyl-(1->4)-6-deoxy-alloside] Formula: C35H52O13 (680.3408) Adducts: [M+H-2H2O]+ (Ppm: 2.8) |
Homo sapiens (esophagus) |
TO40TResolution: 17μm, 82x74
|
|
| m/z_645.3262 Formula: - (n/a) Adducts: (Ppm: ) |
Homo sapiens (colorectal adenocarcinoma) |
240TopL, 210TopR, 230BottomL, 220BottomR-centroidResolution: 50μm, 142x141
|
|
