M/Z: 640.3223

Hit 3 annotations: Binaltorphimine_[M+H-2H2O]+; Protorifamycin I_[M+H]+; N1,N5,N10-Tris-trans-p-coumaroylspermine_[M]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 2 Reference Ions Near m/z 640.3223

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000006330	640.3217	640.3216 ~ 640.3218 MzDiff: `0.5 ppm`	Binaltorphimine (BioDeep_00000176694) Formula: C41H45N3O6 (675.3308)	4.59 (100%)	Rattus norvegicus [UBERON:0004358] caput epididymis
MSI_000022489 Unreliable	640.313	640.313 ~ 640.313 MzDiff: `none`	O-Methylsomniferine (BioDeep_00000035653) Formula: C37H38N2O7 (622.2679)	1.67 (100%)	Mus musculus [UBERON:0004250] upper arm bone

Found 6 Sample Hits

Metabolite	Species	Sample
Binaltorphimine Formula: C41H45N3O6 (675.3308) Adducts: [M+H-2H2O]+ (Ppm: 7.6)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_44 Resolution: 17μm, 299x111 Description
Binaltorphimine Formula: C41H45N3O6 (675.3308) Adducts: [M+H-2H2O]+ (Ppm: 7.2)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_46 Resolution: 17μm, 298x106 Description
Binaltorphimine Formula: C41H45N3O6 (675.3308) Adducts: [M+H-2H2O]+ (Ppm: 7.4)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_47 Resolution: 17μm, 301x111 Description
Binaltorphimine Formula: C41H45N3O6 (675.3308) Adducts: [M+H-2H2O]+ (Ppm: 7.1)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_48 Resolution: 17μm, 294x107 Description
Protorifamycin I Formula: C35H45NO10 (639.3043) Adducts: [M+H]+ (Ppm: 13)	Mytilus edulis (gill)	20190202_MS38_Crassostrea_Gill_350-1500_DHB_pos_A25_11um_305x210 Resolution: 11μm, 305x210 Description single cell layer `class_4` is the gill structure cells, metabolite ion 534.2956 is the top representive ion of this type of cell
N1,N5,N10-Tris-trans-p-coumaroylspermine Formula: C37H44N4O6 (640.3261) Adducts: [M]+ (Ppm: 5)	Drosophila melanogaster (brain)	Drosophila18 Resolution: 5μm, 686x685 Description Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB