M/Z: 600.3582

Hit 3 annotations: LysoPI(18:0/0:0)_[M-H2O+NH4]+; PA(8:0/20:3(5Z,8Z,11Z)-O(14R,15S))_[M-H2O+NH4]+; 4-[3-[3-[Bis[4-(2-methylpropyl)phenyl]methylamino]benzoyl]indol-1-yl]butanoic acid_[M-H2O+NH4]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 3 Reference Ions Near m/z 600.3582

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000024713 Unreliable	600.3645	600.3645 ~ 600.3645 MzDiff: `none`	PA(8:0/20:3(5Z,8Z,11Z)-O(14R,15S)) (BioDeep_00000191579) Formula: C31H53O9P (600.3427)	1.77 (100%)	Mus musculus [UBERON:0004269] upper arm connective tissue
MSI_000057037 Unreliable	600.3504	600.3504 ~ 600.3504 MzDiff: `none`	LysoPI(18:0/0:0) (BioDeep_00000169726) Formula: C27H53O12P (600.3274)	1.44 (100%)	Homo sapiens [UBERON:0007779] transudate
MSI_000057666 Unreliable	600.3582	600.3582 ~ 600.3582 MzDiff: `none`	4-[3-[3-[Bis[4-(2-methylpropyl)phenyl]methylamino]benzoyl]indol-1-yl]butanoic acid (BioDeep_00000179064) Formula: C40H44N2O3 (600.3352)	0.7 (100%)	Homo sapiens [UBERON:0007779] transudate

Found 4 Sample Hits

Metabolite	Species	Sample
LysoPI(18:0/0:0) Formula: C27H53O12P (600.3274) Adducts: [M-H2O+NH4]+ (Ppm: 19.8)	Mus musculus (Lung)	image1 Resolution: 40μm, 187x165 Description Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5
PA(8:0/20:3(5Z,8Z,11Z)-O(14R,15S)) Formula: C31H53O9P (600.3427) Adducts: [M-H2O+NH4]+ (Ppm: 4.9)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.
4-[3-[3-[Bis[4-(2-methylpropyl)phenyl]methylamino]benzoyl]indol-1-yl]butanoic acid Formula: C40H44N2O3 (600.3352) Adducts: [M-H2O+NH4]+ (Ppm: 0.4)	Homo sapiens (esophagus)	LNTO22_1_4 Resolution: 17μm, 82x80 Description
PA(8:0/20:3(5Z,8Z,11Z)-O(14R,15S)) Formula: C31H53O9P (600.3427) Adducts: [M-H2O+NH4]+ (Ppm: 3.6)	Drosophila melanogaster (brain)	Drosophila18 Resolution: 5μm, 686x685 Description Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB