M/Z: 595.2546

Hit 3 annotations: Nonioside B_[M+H-2H2O]+; Brassica napus non-fluorescent chlorophyll catabolite 3_[M+H-2H2O]+; Acrimarine J_[M-H2O+NH4]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 3 Reference Ions Near m/z 595.2546

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000029573 Unreliable	595.2601	595.2601 ~ 595.2601 MzDiff: `none`	Taurocholic acid 3-sulfate (BioDeep_00000027835) Formula: C26H45NO10S2 (595.2485)	0.34 (100%)	Macropus giganteus [UBERON:0002336] corpus callosum
MSI_000031528 Unreliable	595.2601	595.2601 ~ 595.2601 MzDiff: `none`	Taurocholic acid 3-sulfate (BioDeep_00000027835) Formula: C26H45NO10S2 (595.2485)	0.45 (100%)	Macropus giganteus [UBERON:0006093] precuneus cortex
MSI_000031724 Unavailable	595.2546	595.2546 ~ 595.2546 MzDiff: `none`	Brassica napus non-fluorescent chlorophyll catabolite 3 (BioDeep_00000024511) Formula: C34H38N4O8 (630.269)	-0.03 (100%)	Macropus giganteus [UBERON:0006093] precuneus cortex

Found 4 Sample Hits

Metabolite	Species	Sample
Nonioside B Formula: C26H46O17 (630.2735) Adducts: [M+H-2H2O]+ (Ppm: 6)	Mus musculus (Lung)	image3 Resolution: 40μm, 146x190 Description Fig. 4 MALDI-MSI data of mouse lung tissue after administration with D9-choline and U13C-DPPC–containing Poractant alfa surfactant (labels administered 12 h prior to tissue collection). Ion images of (A) m/z 796.6856 ([U13C-DPPC+Na]+), (B) m/z 756.5154 [PC32:0+Na]+), and (C) m/z 765.6079 ([D9-PC32:0+Na]+). D: Overlay image of [U13C-PC32:0+Na]+ (red) and [D9-PC32:0+Na]+ (green). Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC.
Brassica napus non-fluorescent chlorophyll catabolite 3 Formula: C34H38N4O8 (630.269) Adducts: [M+H-2H2O]+ (Ppm: 0.8)	Macropus giganteus (Brain)	170321_kangaroobrain-dan3-pos_maxof50.0_med1 Resolution: 50μm, 81x50 Description Sample information Organism: Macropus giganteus (kangaroo) Organism part: Brain Condition: Wildtype Sample growth conditions: Wild
Brassica napus non-fluorescent chlorophyll catabolite 3 Formula: C34H38N4O8 (630.269) Adducts: [M+H-2H2O]+ (Ppm: 14.3)	Mytilus edulis (gill)	20190202_MS38_Crassostrea_Gill_350-1500_DHB_pos_A25_11um_305x210 Resolution: 11μm, 305x210 Description single cell layer `class_4` is the gill structure cells, metabolite ion 534.2956 is the top representive ion of this type of cell
Acrimarine J Formula: C35H33NO8 (595.2206) Adducts: [M-H2O+NH4]+ (Ppm: 1.7)	Drosophila melanogaster (brain)	Drosophila18 Resolution: 5μm, 686x685 Description Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB