M/Z: 587.54

Hit 1 annotations: DG(18:0/18:1(9Z)/0:0)_[M+H-2H2O]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 2 Reference Ions Near m/z 587.54

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000023664 Unreliable	587.5399	587.5399 ~ 587.5399 MzDiff: `none`	DG(18:0/18:1(9Z)/0:0) (BioDeep_00000028497) Formula: C39H74O5 (622.5536)	1.32 (100%)	Mus musculus [UBERON:0004263] upper arm skin
MSI_000023345 Unreliable	587.547	587.547 ~ 587.547 MzDiff: `none`	Not Annotated	1.75 (%)	Mus musculus [UBERON:0004263] upper arm skin

Found 3 Sample Hits

Metabolite	Species	Sample
DG(18:0/18:1(9Z)/0:0) Formula: C39H74O5 (622.5536) Adducts: [M+H-2H2O]+ (Ppm: 0.3)	Mus musculus (Left upper arm)	357_l_total ion count Resolution: 50μm, 97x131 Description Diseased
DG(18:0/18:1(9Z)/0:0) Formula: C39H74O5 (622.5536) Adducts: [M+H-2H2O]+ (Ppm: 14.4)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.
DG(18:0/18:1(9Z)/0:0) Formula: C39H74O5 (622.5536) Adducts: [M+H-2H2O]+ (Ppm: 0.4)	Drosophila melanogaster (brain)	Drosophila18 Resolution: 5μm, 686x685 Description Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB

Metabolite

Species

Sample

DG(18:0/18:1(9Z)/0:0)

Formula: C39H74O5 (622.5536)
Adducts: [M+H-2H2O]+ (Ppm: 0.3)

Mus musculus (Left upper arm)

357_l_total ion count

Resolution: 50μm, 97x131

Description

Diseased

DG(18:0/18:1(9Z)/0:0)

Formula: C39H74O5 (622.5536)
Adducts: [M+H-2H2O]+ (Ppm: 14.4)

Mus musculus (Lung)

image5

Resolution: 40μm, 163x183

Description

Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.

DG(18:0/18:1(9Z)/0:0)

Formula: C39H74O5 (622.5536)
Adducts: [M+H-2H2O]+ (Ppm: 0.4)

Drosophila melanogaster (brain)

Drosophila18

Resolution: 5μm, 686x685

Description

Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB