M/Z: 577.254

Hit 2 annotations: Benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide_[M+H-2H2O]+; Bafetinib_[M+H]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 3 Reference Ions Near m/z 577.254

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000009902 Unreliable	577.2618	577.2618 ~ 577.2618 MzDiff: `0.1 ppm`	Bafetinib (BioDeep_00000169686) Formula: C30H31F3N8O (576.2573)	3.29 (100%)	Bathymodiolus [UBERON:0009120] gill filament
MSI_000012057 Unavailable	577.2618	577.2618 ~ 577.2618 MzDiff: `none`	Bafetinib (BioDeep_00000169686) Formula: C30H31F3N8O (576.2573)	-0.88 (100%)	Bathymodiolus [UBERON:2000211] gill lamella
MSI_000004674 Unreliable	577.254	577.254 ~ 577.254 MzDiff: `none`	Benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide (BioDeep_00000176601) Formula: C33H36N6O6 (612.2696)	0.33 (100%)	Homo sapiens [UBERON:0002107] liver

Found 2 Sample Hits

Metabolite	Species	Sample
Benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide Formula: C33H36N6O6 (612.2696) Adducts: [M+H-2H2O]+ (Ppm: 3.1)	Homo sapiens (Liver)	20171107_FIT4_DHBpos_p70_s50 Resolution: 50μm, 70x70 Description
Bafetinib Formula: C30H31F3N8O (576.2573) Adducts: [M+H]+ (Ppm: 19.7)	Mus musculus (Lung)	image1 Resolution: 40μm, 187x165 Description Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5

Metabolite

Species

Sample

Benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide

Formula: C33H36N6O6 (612.2696)
Adducts: [M+H-2H2O]+ (Ppm: 3.1)

Homo sapiens (Liver)

20171107_FIT4_DHBpos_p70_s50

Resolution: 50μm, 70x70

Description

Bafetinib

Formula: C30H31F3N8O (576.2573)
Adducts: [M+H]+ (Ppm: 19.7)

Mus musculus (Lung)

image1

Resolution: 40μm, 187x165

Description

Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5