M/Z: 556.0284

Hit 1 annotations: C.I. Direct Red 45_[M+H]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 5 Reference Ions Near m/z 556.0284

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000010996 Unreliable	556.0285	556.0285 ~ 556.0285 MzDiff: `none`	C.I. Direct Red 45 (BioDeep_00000034529) Formula: C24H17N3O7S3 (555.0229)	2.68 (100%)	Mus musculus [UBERON:0012378] muscle layer of urinary bladder
MSI_000011003 Unreliable	556.0372	556.0372 ~ 556.0372 MzDiff: `none`	trans-zeatin riboside triphosphate (BioDeep_00000011186) Formula: C15H24N5O14P3 (591.0533)	2.65 (100%)	Mus musculus [UBERON:0012378] muscle layer of urinary bladder
MSI_000009499 Unavailable	556.0285	556.0285 ~ 556.0285 MzDiff: `none`	C.I. Direct Red 45 (BioDeep_00000034529) Formula: C24H17N3O7S3 (555.0229)	-0.59 (100%)	Mus musculus [UBERON:0004645] urinary bladder urothelium
MSI_000009553 Unavailable	556.0372	556.0372 ~ 556.0372 MzDiff: `none`	trans-zeatin riboside triphosphate (BioDeep_00000011186) Formula: C15H24N5O14P3 (591.0533)	-0.89 (100%)	Mus musculus [UBERON:0004645] urinary bladder urothelium
MSI_000024963 Unreliable	556.0284	556.0284 ~ 556.0284 MzDiff: `none`	C.I. Direct Red 45 (BioDeep_00000034529) Formula: C24H17N3O7S3 (555.0229)	1.75 (100%)	Mus musculus [UBERON:0004269] upper arm connective tissue

Found 2 Sample Hits

Metabolite	Species	Sample
C.I. Direct Red 45 Formula: C24H17N3O7S3 (555.0229) Adducts: [M+H]+ (Ppm: 2.9)	Mus musculus (Urinary bladder)	HR2MSI_mouse_urinary_bladder - S096 Resolution: 10μm, 260x134 Description Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset) The spatial distribution of phospholipids in a tissue section of mouse urinary bladder was analyzed by MALDI MS imaging at 10 micrometer pixel size with high mass resolution (using an LTQ Orbitrap mass spectrometer). R, ö, mpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B, Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl, 49(22):3834-8(2010) Fig. S2: Single ion images of compounds shown in Fig. 1A-B : (upper left to lower right) m/z = 743.5482 (unknown), m/z = 741.5307 (SM (16:0), [M+K]+), m/z = 798.5410 (PC (34:1), [M+K]+), m/z = 616.1767 (heme b, M+), m/z = 772.5253 (PC (32:0), [M+K]+). Stability of determined mass values was in the range of +/- 1 ppm over 22 hours of measurement (Fig. S4), with a standard deviation of 0.56 ppm. Accuracy data were obtained during tissue scanning experiments by monitoring the mass signal at nominal mass 798. The internal lock mass function of the Orbitrap instrument was used for automatic calibration during imaging measurements, using the known matrix-related ion signals at m/z = 137.0233, m/z = 444.0925 and m/z = 716.1246.
C.I. Direct Red 45 Formula: C24H17N3O7S3 (555.0229) Adducts: [M+H]+ (Ppm: 3.1)	Mus musculus (Left upper arm)	357_l_total ion count Resolution: 50μm, 97x131 Description Diseased

Metabolite

Species

Sample

C.I. Direct Red 45

Formula: C24H17N3O7S3 (555.0229)
Adducts: [M+H]+ (Ppm: 2.9)

Mus musculus (Urinary bladder)

HR2MSI_mouse_urinary_bladder - S096

Resolution: 10μm, 260x134

Description

Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset)
The spatial distribution of phospholipids in a tissue section of mouse urinary bladder was analyzed by MALDI MS imaging at 10 micrometer pixel size with high mass resolution (using an LTQ Orbitrap mass spectrometer).

R, ö, mpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B, Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl, 49(22):3834-8(2010)

Fig. S2: Single ion images of compounds shown in Fig. 1A-B : (upper left to lower right) m/z = 743.5482 (unknown), m/z = 741.5307 (SM (16:0), [M+K]+), m/z = 798.5410 (PC (34:1), [M+K]+), m/z = 616.1767 (heme b, M+), m/z = 772.5253 (PC (32:0), [M+K]+).

Stability of determined mass values was in the range of +/- 1 ppm over 22 hours of measurement (Fig. S4), with a standard deviation of 0.56 ppm. Accuracy data were obtained during tissue scanning experiments by monitoring the mass signal at nominal mass 798. The internal lock mass function of the Orbitrap instrument was used for automatic calibration during imaging measurements, using the known matrix-related ion signals at m/z = 137.0233, m/z = 444.0925 and m/z = 716.1246.

C.I. Direct Red 45

Formula: C24H17N3O7S3 (555.0229)
Adducts: [M+H]+ (Ppm: 3.1)

Mus musculus (Left upper arm)

357_l_total ion count

Resolution: 50μm, 97x131

Description

Diseased