M/Z: 533.2341

Hit 2 annotations: Alpinnanin B_[M+H-H2O]+; Nomilinic acid_[M+H]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 6 Reference Ions Near m/z 533.2341

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000011558 Unavailable	533.2343	533.2343 ~ 533.2343 MzDiff: `none`	Alpinnanin B (BioDeep_00001029507) Formula: C35H34O6 (550.2355)	-0.85 (100%)	Mus musculus [UBERON:0012378] muscle layer of urinary bladder
MSI_000011591 Unavailable	533.2425	533.2425 ~ 533.2425 MzDiff: `none`	Nomilinic acid (BioDeep_00000020231) Formula: C28H36O10 (532.2308)	-1.2 (100%)	Mus musculus [UBERON:0012378] muscle layer of urinary bladder
MSI_000009805 Unavailable	533.2343	533.2343 ~ 533.2343 MzDiff: `none`	Alpinnanin B (BioDeep_00001029507) Formula: C35H34O6 (550.2355)	-1.7 (100%)	Mus musculus [UBERON:0004645] urinary bladder urothelium
MSI_000009687 Unavailable	533.2425	533.2425 ~ 533.2425 MzDiff: `none`	Nomilinic acid (BioDeep_00000020231) Formula: C28H36O10 (532.2308)	-1.4 (100%)	Mus musculus [UBERON:0004645] urinary bladder urothelium
MSI_000000276 Unreliable	533.2343	533.2343 ~ 533.2343 MzDiff: `none`	Alpinnanin B (BioDeep_00001029507) Formula: C35H34O6 (550.2355)	1.1 (100%)	Mus musculus [CL:0000066] epithelial cell
MSI_000000326 Unreliable	533.2425	533.2425 ~ 533.2425 MzDiff: `none`	Nomilinic acid (BioDeep_00000020231) Formula: C28H36O10 (532.2308)	0.77 (100%)	Mus musculus [CL:0000066] epithelial cell

Found 3 Sample Hits

Metabolite	Species	Sample
Alpinnanin B Formula: C35H34O6 (550.2355) Adducts: [M+H-H2O]+ (Ppm: 3.9)	Mus musculus (Urinary bladder)	HR2MSI_mouse_urinary_bladder - S096 Resolution: 10μm, 260x134 Description Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset) The spatial distribution of phospholipids in a tissue section of mouse urinary bladder was analyzed by MALDI MS imaging at 10 micrometer pixel size with high mass resolution (using an LTQ Orbitrap mass spectrometer). R, ö, mpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B, Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl, 49(22):3834-8(2010) Fig. S2: Single ion images of compounds shown in Fig. 1A-B : (upper left to lower right) m/z = 743.5482 (unknown), m/z = 741.5307 (SM (16:0), [M+K]+), m/z = 798.5410 (PC (34:1), [M+K]+), m/z = 616.1767 (heme b, M+), m/z = 772.5253 (PC (32:0), [M+K]+). Stability of determined mass values was in the range of +/- 1 ppm over 22 hours of measurement (Fig. S4), with a standard deviation of 0.56 ppm. Accuracy data were obtained during tissue scanning experiments by monitoring the mass signal at nominal mass 798. The internal lock mass function of the Orbitrap instrument was used for automatic calibration during imaging measurements, using the known matrix-related ion signals at m/z = 137.0233, m/z = 444.0925 and m/z = 716.1246.
Nomilinic acid Formula: C28H36O10 (532.2308) Adducts: [M+H]+ (Ppm: 6.2)	Macropus giganteus (Brain)	170321_kangaroobrain-dan3-pos_maxof50.0_med1 Resolution: 50μm, 81x50 Description Sample information Organism: Macropus giganteus (kangaroo) Organism part: Brain Condition: Wildtype Sample growth conditions: Wild
Nomilinic acid Formula: C28H36O10 (532.2308) Adducts: [M+H]+ (Ppm: 7.5)	Drosophila melanogaster (brain)	Drosophila18 Resolution: 5μm, 686x685 Description Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB

Metabolite

Species

Sample

Alpinnanin B

Formula: C35H34O6 (550.2355)
Adducts: [M+H-H2O]+ (Ppm: 3.9)

Mus musculus (Urinary bladder)

HR2MSI_mouse_urinary_bladder - S096

Resolution: 10μm, 260x134

Description

Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset)
The spatial distribution of phospholipids in a tissue section of mouse urinary bladder was analyzed by MALDI MS imaging at 10 micrometer pixel size with high mass resolution (using an LTQ Orbitrap mass spectrometer).

R, ö, mpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B, Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl, 49(22):3834-8(2010)

Fig. S2: Single ion images of compounds shown in Fig. 1A-B : (upper left to lower right) m/z = 743.5482 (unknown), m/z = 741.5307 (SM (16:0), [M+K]+), m/z = 798.5410 (PC (34:1), [M+K]+), m/z = 616.1767 (heme b, M+), m/z = 772.5253 (PC (32:0), [M+K]+).

Stability of determined mass values was in the range of +/- 1 ppm over 22 hours of measurement (Fig. S4), with a standard deviation of 0.56 ppm. Accuracy data were obtained during tissue scanning experiments by monitoring the mass signal at nominal mass 798. The internal lock mass function of the Orbitrap instrument was used for automatic calibration during imaging measurements, using the known matrix-related ion signals at m/z = 137.0233, m/z = 444.0925 and m/z = 716.1246.

Nomilinic acid

Formula: C28H36O10 (532.2308)
Adducts: [M+H]+ (Ppm: 6.2)

Macropus giganteus (Brain)

170321_kangaroobrain-dan3-pos_maxof50.0_med1

Resolution: 50μm, 81x50

Description

Sample information Organism: Macropus giganteus (kangaroo) Organism part: Brain Condition: Wildtype Sample growth conditions: Wild

Nomilinic acid

Formula: C28H36O10 (532.2308)
Adducts: [M+H]+ (Ppm: 7.5)

Drosophila melanogaster (brain)

Drosophila18

Resolution: 5μm, 686x685

Description

Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB