M/Z: 527.0183

Hit 2 annotations: (17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one_[M+H-2H2O]+; J Acid urea_[M+Na]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 8 Reference Ions Near m/z 527.0183

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000017267 Reliable	527.0175	527.0174 ~ 527.0175 MzDiff: `0.1 ppm`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	3.87 (100%)	Vitis vinifera [PO:0009087] mesocarp
MSI_000016433 Unreliable	527.0175	527.0175 ~ 527.0175 MzDiff: `0.0 ppm`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	1.01 (100%)	Vitis vinifera [PO:0009086] endocarp
MSI_000012948 Unavailable	527.0175	527.0175 ~ 527.0175 MzDiff: `none`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	-0.56 (100%)	Plant [PO:0005020] vascular bundle
MSI_000013908 Unavailable	527.0175	527.0175 ~ 527.0175 MzDiff: `none`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	-0.06 (100%)	Plant [PO:0005417] phloem
MSI_000015147 Unavailable	527.0175	527.0175 ~ 527.0175 MzDiff: `none`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	-0.59 (100%)	Plant [PO:0006036] root epidermis
MSI_000018509 Unreliable	527.0175	527.0175 ~ 527.0175 MzDiff: `none`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	1.75 (100%)	Plant [PO:0020124] root stele
MSI_000020161 Unavailable	527.0175	527.0175 ~ 527.0175 MzDiff: `none`	(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one (BioDeep_00002237633) Formula: C27H21Cl3O7 (562.0353)	-0.54 (100%)	Plant [PO:0025197] stele
MSI_000024650 Unreliable	527.0183	527.0183 ~ 527.0183 MzDiff: `none`	J Acid urea (BioDeep_00000172776) Formula: C21H16N2O9S2 (504.0297)	1.77 (100%)	Mus musculus [UBERON:0004269] upper arm connective tissue

Found 6 Sample Hits

Metabolite	Species	Sample
(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one Formula: C27H21Cl3O7 (562.0353) Adducts: [M+H-2H2O]+ (Ppm: 7.5)	Plant (Root)	MPIMM_035_QE_P_PO_6pm Resolution: 30μm, 165x170 Description
(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one Formula: C27H21Cl3O7 (562.0353) Adducts: [M+H-2H2O]+ (Ppm: 7.5)	Vitis vinifera (Fruit)	grape_dhb_91_1 Resolution: 50μm, 120x114 Description Grape berries fruit, condition: Ripe
(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one Formula: C27H21Cl3O7 (562.0353) Adducts: [M+H-2H2O]+ (Ppm: 7.5)	Vitis vinifera (Fruit)	grape_dhb_164_1 Resolution: 17μm, 136x122 Description Grape berries fruit, condition: Late
(17r)-8,10,20-trichloro-3,7,9,21-tetrahydroxy-12,12,17,23-tetramethyl-18,25-dioxahexacyclo[15.7.1.0²,¹⁵.0⁴,¹³.0⁶,¹¹.0¹⁹,²⁴]pentacosa-2,4(13),6,8,10,14,19(24),20,22-nonaen-5-one Formula: C27H21Cl3O7 (562.0353) Adducts: [M+H-2H2O]+ (Ppm: 7.6)	Vitis vinifera (Fruit)	grape_dhb_163_1 Resolution: 17μm, 132x115 Description Grape berries fruit, condition: Late
J Acid urea Formula: C21H16N2O9S2 (504.0297) Adducts: [M+Na]+ (Ppm: 1.2)	Mus musculus (Left upper arm)	357_l_total ion count Resolution: 50μm, 97x131 Description Diseased
J Acid urea Formula: C21H16N2O9S2 (504.0297) Adducts: [M+Na]+ (Ppm: 0.3)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.