M/Z: 478.3385
Hit 3 annotations: Hexosylsphingosine_[M+H]+
; Gentamicin_[M+H]+
; LysoPC(16:0/0:0)_[M+H-H2O]+
- Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
- Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
- Unreliable: 这个参考离子具有较高的排名价值,但缺乏可重复性。
- Unavailable: 由于排名价值低且缺乏可重复性,这个参考离子不应用于注释。
Found 30 Reference Ions Near m/z 478.3385
NovoCell ID | m/z | Mass Window | Metabolite | Ranking | Anatomy Context |
---|---|---|---|---|---|
MSI_000006399 Reliable | 478.3293 | 478.3292 ~ 478.3294 MzDiff: 0.7 ppm |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
6.76 (100%) | Rattus norvegicus [UBERON:0004358] caput epididymis |
MSI_000011625 Unavailable | 478.3339 | 478.3339 ~ 478.3339 MzDiff: none |
Not Annotated | -1.57 (%) | Mus musculus [UBERON:0012378] muscle layer of urinary bladder |
MSI_000011660 Unavailable | 478.3396 | 478.3396 ~ 478.3396 MzDiff: none |
Hexosylsphingosine (BioDeep_00000179768) Formula: C24H47NO8 (477.3302) |
-1.92 (100%) | Mus musculus [UBERON:0012378] muscle layer of urinary bladder |
MSI_000026412 Unreliable | 478.3287 | 478.3282 ~ 478.3289 MzDiff: 2.9 ppm |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
3.98 (100%) | Mus musculus [UBERON:0002048] lung |
MSI_000010684 Unavailable | 478.3294 | 478.3294 ~ 478.3294 MzDiff: 0.0 ppm |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
-2.65 (100%) | Bathymodiolus [UBERON:0009120] gill filament |
MSI_000061448 Unreliable | 478.3295 | 478.3295 ~ 478.3295 MzDiff: 0.0 ppm |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
1.81 (100%) | Mus musculus [UBERON:0000956] cerebral cortex |
MSI_000012266 Unavailable | 478.3294 | 478.3294 ~ 478.3294 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
-1.11 (100%) | Bathymodiolus [UBERON:2000211] gill lamella |
MSI_000058572 Unreliable | 478.3294 | 478.3292 ~ 478.3295 MzDiff: 1.3 ppm |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
2.22 (100%) | Mus musculus [UBERON:0001950] neocortex |
MSI_000021782 Unreliable | 478.3303 | 478.3303 ~ 478.3303 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
0.65 (100%) | Mus musculus [UBERON:0001499] muscle of arm |
MSI_000009474 Unavailable | 478.3339 | 478.3339 ~ 478.3339 MzDiff: none |
Not Annotated | -0.5 (%) | Mus musculus [UBERON:0004645] urinary bladder urothelium |
MSI_000009336 Unreliable | 478.3396 | 478.3396 ~ 478.3396 MzDiff: none |
Hexosylsphingosine (BioDeep_00000179768) Formula: C24H47NO8 (477.3302) |
(100%) | Mus musculus [UBERON:0004645] urinary bladder urothelium |
MSI_000033869 Unreliable | 478.3288 | 478.3288 ~ 478.3288 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
0.35 (100%) | Posidonia oceanica [PO:0005352] xylem |
MSI_000034951 Unavailable | 478.3288 | 478.3288 ~ 478.3288 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
-0.33 (100%) | Posidonia oceanica [PO:0006036] root epidermis |
MSI_000049989 Unreliable | 478.3291 | 478.3291 ~ 478.3291 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
1.26 (100%) | Mytilus edulis [UBERON:0009120] gill filament |
MSI_000051094 Unreliable | 478.3291 | 478.3291 ~ 478.3291 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
0.04 (100%) | Mytilus edulis [UBERON:2001856] gill ray |
MSI_000059939 Unavailable | 478.3292 | 478.3292 ~ 478.3292 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
-0.73 (100%) | Mus musculus [UBERON:0002298] brainstem |
MSI_000060678 Unavailable | 478.3292 | 478.3292 ~ 478.3292 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
-0.41 (100%) | Mus musculus [UBERON:0002421] hippocampal formation |
MSI_000043969 Unreliable | 478.3292 | 478.3292 ~ 478.3292 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
2.23 (100%) | Rattus norvegicus [UBERON:0002264] olfactory bulb |
MSI_000032133 Unreliable | 478.3297 | 478.3297 ~ 478.3297 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
1.5 (100%) | Posidonia oceanica [PO:0005020] vascular bundle |
MSI_000013327 Unreliable | 478.3299 | 478.3299 ~ 478.3299 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
1.59 (100%) | Plant [PO:0005417] phloem |
MSI_000015024 Unavailable | 478.3299 | 478.3299 ~ 478.3299 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
-0.53 (100%) | Plant [PO:0006036] root epidermis |
MSI_000018983 Unavailable | 478.3299 | 478.3299 ~ 478.3299 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
-0.06 (100%) | Plant [PO:0020124] root stele |
MSI_000019629 Unreliable | 478.3299 | 478.3299 ~ 478.3299 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
0.09 (100%) | Plant [PO:0025197] stele |
MSI_000002486 Unreliable | 478.3321 | 478.3321 ~ 478.3321 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
0.94 (100%) | Rattus norvegicus [UBERON:0001950] neocortex |
MSI_000003117 Unreliable | 478.3321 | 478.3321 ~ 478.3321 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
1.99 (100%) | Rattus norvegicus [UBERON:0002037] cerebellum |
MSI_000005076 Unavailable | 478.3321 | 478.3321 ~ 478.3321 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
-0.48 (100%) | Rattus norvegicus [UBERON:0002298] brainstem |
MSI_000005951 Unavailable | 478.3321 | 478.3321 ~ 478.3321 MzDiff: none |
Gentamicin (BioDeep_00000006570) Formula: C21H43N5O7 (477.3162) |
-1.07 (100%) | Rattus norvegicus [UBERON:0002435] striatum |
MSI_000000542 Unavailable | 478.3339 | 478.3339 ~ 478.3339 MzDiff: none |
Not Annotated | -0.4 (%) | Mus musculus [CL:0000066] epithelial cell |
MSI_000057183 Unreliable | 478.3385 | 478.3385 ~ 478.3385 MzDiff: none |
LysoPC(16:0/0:0) (BioDeep_00000018630) Formula: C24H50NO7P (495.3325) |
1.31 (100%) | Homo sapiens [UBERON:0007779] transudate |
MSI_000000609 Unavailable | 478.3396 | 478.3396 ~ 478.3396 MzDiff: none |
Hexosylsphingosine (BioDeep_00000179768) Formula: C24H47NO8 (477.3302) |
-0.5 (100%) | Mus musculus [CL:0000066] epithelial cell |
Found 6 Sample Hits
Metabolite | Species | Sample | |
---|---|---|---|
Hexosylsphingosine Formula: C24H47NO8 (477.3302) Adducts: [M+H]+ (Ppm: 4.5) |
Mus musculus (Urinary bladder) |
HR2MSI_mouse_urinary_bladder - S096Resolution: 10μm, 260x134
Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset) |
|
Gentamicin Formula: C21H43N5O7 (477.3162) Adducts: [M+H]+ (Ppm: 11.1) |
Mus musculus (Lung) |
image1Resolution: 40μm, 187x165
Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin.
Fig 1-3, Fig S1-S3, S5 |
|
Gentamicin Formula: C21H43N5O7 (477.3162) Adducts: [M+H]+ (Ppm: 9.8) |
Mus musculus (Lung) |
image4Resolution: 40μm, 162x156
Fig 6c
Fig. 6 MALDI-MSI of U13C-PC16:0/16:0 acyl chain remodeling. A: Averaged MALDI mass spectrum from lung tissue collected from mice euthanized 12 h after administration of D9-choline and U13C-DPPC–containing Poractant alfa surfactant. The ion at m/z 828.6321 is assigned as the [M+Na]+ ion of 13C24-PC16:0_20:4 formed by acyl remodeling of U13C-PC16:0/16:0. The “NL” value refers to the intensity of the base peak in the full range MS1 spectrum. B: MS/MS spectrum of precursor ions at m/z 828.5 ± 0.5 with fragment ions originating from [13C24-PC16:0_20:4+Na]+ annotated. Part-per-million (ppm) mass errors are provided in parentheses. C, D: MALDI-MSI data of [U13C-DPPC+Na]+ (blue), [PC36:4+Na]+ (green) and [13C24-PC16:0_20:4+Na]+ (red) in lung tissue collected from mice (C) 12 h and (D) 18 h after label administration. All images were visualized using total-ion-current normalization and hotspot removal (high quantile = 99%). MS/MS, tandem mass spectrometry; MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC. |
|
Gentamicin Formula: C21H43N5O7 (477.3162) Adducts: [M+H]+ (Ppm: 11.3) |
Mus musculus (Lung) |
image2Resolution: 40μm, 550x256
Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b)
[PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9-
choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in
parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in
white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar
regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids
PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using
total ion current normalisation and hotspot removal (high quantile = 99%). |
|
LysoPC(16:0/0:0) Formula: C24H50NO7P (495.3325) Adducts: [M+H-H2O]+ (Ppm: 19.5) |
Homo sapiens (esophagus) |
LNTO22_1_4Resolution: 17μm, 82x80
|
|
LysoPC(16:0/0:0) Formula: C24H50NO7P (495.3325) Adducts: [M+H-H2O]+ (Ppm: ) |
Mus musculus (brain) |
Brain01_Bregma-3-88b_centroidResolution: 17μm, 265x320
|
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