M/Z: 478.3339


Hit 2 annotations:  LysoPC(16:0/0:0)_[M+H-H2O]+; Gentamicin_[M+H]+


在BioDeep NovoCell知识数据库中,参考离子总共被划分为4个级别。
  • Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
  • Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
  • Unreliable: 这个参考离子具有较高的排名价值,但缺乏可重复性。
  • Unavailable: 由于排名价值低且缺乏可重复性,这个参考离子不应用于注释。

Found 30 Reference Ions Near m/z 478.3339
NovoCell ID m/z Mass Window Metabolite Ranking Anatomy Context
MSI_000006399 Reliable 478.3293 478.3292 ~ 478.3294
MzDiff: 0.7 ppm
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
6.76 (100%) Rattus norvegicus
[UBERON:0004358] caput epididymis
MSI_000011660 Unavailable 478.3396 478.3396 ~ 478.3396
MzDiff: none
Hexosylsphingosine (BioDeep_00000179768)
Formula: C24H47NO8 (477.3302)
-1.92 (100%) Mus musculus
[UBERON:0012378] muscle layer of urinary bladder
MSI_000011625 Unavailable 478.3339 478.3339 ~ 478.3339
MzDiff: none
Not Annotated -1.57 (%) Mus musculus
[UBERON:0012378] muscle layer of urinary bladder
MSI_000011621 Unavailable 478.3277 478.3277 ~ 478.3277
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-1.52 (100%) Mus musculus
[UBERON:0012378] muscle layer of urinary bladder
MSI_000010684 Unavailable 478.3294 478.3294 ~ 478.3294
MzDiff: 0.0 ppm
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
-2.65 (100%) Bathymodiolus
[UBERON:0009120] gill filament
MSI_000026412 Unreliable 478.3287 478.3282 ~ 478.3289
MzDiff: 2.9 ppm
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
3.98 (100%) Mus musculus
[UBERON:0002048] lung
MSI_000061448 Unreliable 478.3295 478.3295 ~ 478.3295
MzDiff: 0.0 ppm
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
1.81 (100%) Mus musculus
[UBERON:0000956] cerebral cortex
MSI_000009496 Unavailable 478.3277 478.3277 ~ 478.3277
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.57 (100%) Mus musculus
[UBERON:0004645] urinary bladder urothelium
MSI_000012266 Unavailable 478.3294 478.3294 ~ 478.3294
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
-1.11 (100%) Bathymodiolus
[UBERON:2000211] gill lamella
MSI_000021782 Unreliable 478.3303 478.3303 ~ 478.3303
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
0.65 (100%) Mus musculus
[UBERON:0001499] muscle of arm
MSI_000009474 Unavailable 478.3339 478.3339 ~ 478.3339
MzDiff: none
Not Annotated -0.5 (%) Mus musculus
[UBERON:0004645] urinary bladder urothelium
MSI_000058572 Unreliable 478.3294 478.3292 ~ 478.3295
MzDiff: 1.3 ppm
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
2.22 (100%) Mus musculus
[UBERON:0001950] neocortex
MSI_000009336 Unreliable 478.3396 478.3396 ~ 478.3396
MzDiff: none
Hexosylsphingosine (BioDeep_00000179768)
Formula: C24H47NO8 (477.3302)
(100%) Mus musculus
[UBERON:0004645] urinary bladder urothelium
MSI_000001655 Unavailable 478.3267 478.3267 ~ 478.3267
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
-0.48 (100%) Mus musculus
[UBERON:0001225] cortex of kidney
MSI_000002215 Unavailable 478.3267 478.3267 ~ 478.3267
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
-0.56 (100%) Mus musculus
[UBERON:0001293] outer medulla of kidney
MSI_000000537 Unavailable 478.3277 478.3277 ~ 478.3277
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.38 (100%) Mus musculus
[CL:0000066] epithelial cell
MSI_000033869 Unreliable 478.3288 478.3288 ~ 478.3288
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
0.35 (100%) Posidonia oceanica
[PO:0005352] xylem
MSI_000034951 Unavailable 478.3288 478.3288 ~ 478.3288
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.33 (100%) Posidonia oceanica
[PO:0006036] root epidermis
MSI_000049989 Unreliable 478.3291 478.3291 ~ 478.3291
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
1.26 (100%) Mytilus edulis
[UBERON:0009120] gill filament
MSI_000051094 Unreliable 478.3291 478.3291 ~ 478.3291
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
0.04 (100%) Mytilus edulis
[UBERON:2001856] gill ray
MSI_000059939 Unavailable 478.3292 478.3292 ~ 478.3292
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.73 (100%) Mus musculus
[UBERON:0002298] brainstem
MSI_000060678 Unavailable 478.3292 478.3292 ~ 478.3292
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.41 (100%) Mus musculus
[UBERON:0002421] hippocampal formation
MSI_000043969 Unreliable 478.3292 478.3292 ~ 478.3292
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
2.23 (100%) Rattus norvegicus
[UBERON:0002264] olfactory bulb
MSI_000032133 Unreliable 478.3297 478.3297 ~ 478.3297
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
1.5 (100%) Posidonia oceanica
[PO:0005020] vascular bundle
MSI_000013327 Unreliable 478.3299 478.3299 ~ 478.3299
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
1.59 (100%) Plant
[PO:0005417] phloem
MSI_000015024 Unavailable 478.3299 478.3299 ~ 478.3299
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.53 (100%) Plant
[PO:0006036] root epidermis
MSI_000018983 Unavailable 478.3299 478.3299 ~ 478.3299
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
-0.06 (100%) Plant
[PO:0020124] root stele
MSI_000019629 Unreliable 478.3299 478.3299 ~ 478.3299
MzDiff: none
LysoPC(16:0/0:0) (BioDeep_00000018630)
Formula: C24H50NO7P (495.3325)
0.09 (100%) Plant
[PO:0025197] stele
MSI_000002486 Unreliable 478.3321 478.3321 ~ 478.3321
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
0.94 (100%) Rattus norvegicus
[UBERON:0001950] neocortex
MSI_000003117 Unreliable 478.3321 478.3321 ~ 478.3321
MzDiff: none
Gentamicin (BioDeep_00000006570)
Formula: C21H43N5O7 (477.3162)
1.99 (100%) Rattus norvegicus
[UBERON:0002037] cerebellum

Found 16 Sample Hits
Metabolite Species Sample
m/z_478.3339

Formula: - (n/a)
Adducts: (Ppm: )
Mus musculus (Urinary bladder)
HR2MSI_mouse_urinary_bladder - S096
Resolution: 10μm, 260x134

Description

Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset)
The spatial distribution of phospholipids in a tissue section of mouse urinary bladder was analyzed by MALDI MS imaging at 10 micrometer pixel size with high mass resolution (using an LTQ Orbitrap mass spectrometer).

R, ö, mpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B, Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl, 49(22):3834-8(2010)

Fig. S2: Single ion images of compounds shown in Fig. 1A-B : (upper left to lower right) m/z = 743.5482 (unknown), m/z = 741.5307 (SM (16:0), [M+K]+), m/z = 798.5410 (PC (34:1), [M+K]+), m/z = 616.1767 (heme b, M+), m/z = 772.5253 (PC (32:0), [M+K]+).

Stability of determined mass values was in the range of +/- 1 ppm over 22 hours of measurement (Fig. S4), with a standard deviation of 0.56 ppm. Accuracy data were obtained during tissue scanning experiments by monitoring the mass signal at nominal mass 798. The internal lock mass function of the Orbitrap instrument was used for automatic calibration during imaging measurements, using the known matrix-related ion signals at m/z = 137.0233, m/z = 444.0925 and m/z = 716.1246.

LysoPC(16:0/0:0)

Formula: C24H50NO7P (495.3325)
Adducts: [M+H-H2O]+ (Ppm: 1.5)
Plant (Root)
MPIMM_035_QE_P_PO_6pm
Resolution: 30μm, 165x170

Description

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 18)
Rattus norvegicus (Brain)
Spectroswiss - sol_2x_br_2
Resolution: 17μm, 488x193

Description

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 11.1)
Mus musculus (Lung)
image1
Resolution: 40μm, 187x165

Description

Fig. 2 MALDI-MSI data from the same mouse lung tissue analyzed in Fig. 1. A: Optical image of the post-MSI, H&E-stained tissue section. B–D, F–G: Ion images of (B) m/z 796.6855 ([U13C-DPPC+Na]+), (C) m/z 756.5514 ([PC32:0+Na]+), (D) m/z 765.6079 ([D9-PC32:0+Na]+), (F) m/z 754.5359 ([PC32:1+Na]+), and (G) m/z 763.5923 ([D9-PC32:1+Na]+). E, H: Ratio images of (E) [D9-PC32:0+Na]+:[PC32:0+Na]+ and (H) [D9-PC32:1+Na]+:[PC32:1+Na]+. Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. U13C-DPPC, universally 13C-labeled dipalmitoyl PC; PC, phosphatidylcholine; MSI, mass spectrometry imaging; H&E, hematoxylin and eosin. Fig 1-3, Fig S1-S3, S5

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 14.2)
Mus musculus (Left upper arm)
357_l_total ion count
Resolution: 50μm, 97x131

Description

Diseased

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 10.6)
Mus musculus (Lung)
image3
Resolution: 40μm, 146x190

Description

Fig. 4 MALDI-MSI data of mouse lung tissue after administration with D9-choline and U13C-DPPC–containing Poractant alfa surfactant (labels administered 12 h prior to tissue collection). Ion images of (A) m/z 796.6856 ([U13C-DPPC+Na]+), (B) m/z 756.5154 [PC32:0+Na]+), and (C) m/z 765.6079 ([D9-PC32:0+Na]+). D: Overlay image of [U13C-PC32:0+Na]+ (red) and [D9-PC32:0+Na]+ (green). Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC.

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 9.8)
Mus musculus (Lung)
image4
Resolution: 40μm, 162x156

Description

Fig 6c Fig. 6 MALDI-MSI of U13C-PC16:0/16:0 acyl chain remodeling. A: Averaged MALDI mass spectrum from lung tissue collected from mice euthanized 12 h after administration of D9-choline and U13C-DPPC–containing Poractant alfa surfactant. The ion at m/z 828.6321 is assigned as the [M+Na]+ ion of 13C24-PC16:0_20:4 formed by acyl remodeling of U13C-PC16:0/16:0. The “NL” value refers to the intensity of the base peak in the full range MS1 spectrum. B: MS/MS spectrum of precursor ions at m/z 828.5 ± 0.5 with fragment ions originating from [13C24-PC16:0_20:4+Na]+ annotated. Part-per-million (ppm) mass errors are provided in parentheses. C, D: MALDI-MSI data of [U13C-DPPC+Na]+ (blue), [PC36:4+Na]+ (green) and [13C24-PC16:0_20:4+Na]+ (red) in lung tissue collected from mice (C) 12 h and (D) 18 h after label administration. All images were visualized using total-ion-current normalization and hotspot removal (high quantile = 99%). MS/MS, tandem mass spectrometry; MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC.

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 11.3)
Mus musculus (Lung)
image5
Resolution: 40μm, 163x183

Description

Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 11.3)
Mus musculus (Lung)
image2
Resolution: 40μm, 550x256

Description

Supplementary Figure S6. Ion distribution images for (a) [PC36:4+Na]+ (m/z 804.5514) and (b) [PC38:6+Na]+ (m/z 828.5515) obtained from mouse lung tissue collected 6 h after administration of D9- choline and U13C-DPPC–containing CHF5633. Parts-per-million (ppm) mass errors are indicated in parentheses. (c) Magnification of the boxed region in (a) with selected bronchiolar regions outlined in white boxes. (d) The corresponding H&E-stained tissue section with the same selected bronchiolar regions outlined in black boxes. These data demonstrate the co-localisation of the polyunsaturated lipids PC36:4 and PC38:6 with the bronchiolar regions of the lung. All MSI images were visualised using total ion current normalisation and hotspot removal (high quantile = 99%).

LysoPC(16:0/0:0)

Formula: C24H50NO7P (495.3325)
Adducts: [M+H-H2O]+ (Ppm: 1.1)
Posidonia oceanica (root)
20190613_MS1_A19r-18
Resolution: 17μm, 246x264

Description

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 11.7)
Mytilus edulis (gill)
20190202_MS38_Crassostrea_Gill_350-1500_DHB_pos_A25_11um_305x210
Resolution: 11μm, 305x210

Description

single cell layer class_4 is the gill structure cells, metabolite ion 534.2956 is the top representive ion of this type of cell

Gentamicin

Formula: C21H43N5O7 (477.3162)
Adducts: [M+H]+ (Ppm: 12.1)
Mytilus edulis (mantle)
20190216_MS38_Mytilus_mantle_350-1500_DHB_pos_A26_10um_275x210
Resolution: 10μm, 275x210

Description

LysoPC(16:0/0:0)

Formula: C24H50NO7P (495.3325)
Adducts: [M+H-H2O]+ (Ppm: )
Mus musculus (brain)
Brain01_Bregma-3-88b_centroid
Resolution: 17μm, 265x320

Description

LysoPC(16:0/0:0)

Formula: C24H50NO7P (495.3325)
Adducts: [M+H-H2O]+ (Ppm: 0.7)
Mus musculus (brain)
Brain01_Bregma1-42_02_centroid
Resolution: 17μm, 434x258

Description

LysoPC(16:0/0:0)

Formula: C24H50NO7P (495.3325)
Adducts: [M+H-H2O]+ (Ppm: 0.7)
Mus musculus (brain)
Brain01_Bregma1-42_01_centroid
Resolution: 17μm, 447x118

Description

LysoPC(16:0/0:0)

Formula: C24H50NO7P (495.3325)
Adducts: [M+H-H2O]+ (Ppm: 0.2)
Drosophila melanogaster (brain)
Drosophila18
Resolution: 5μm, 686x685

Description

Sample information Organism: Drosophila melanogaster Organism part: Brain Condition: Healthy Sample preparation Sample stabilisation: Frozen Tissue modification: Frozen MALDI matrix: 2,5-dihydroxybenzoic acid (DHB) MALDI matrix application: TM sprayer Solvent: Aceton/water MS analysis Polarity: Positive Ionisation source: Prototype Analyzer: Orbitrap Pixel size: 5μm × 5μm Annotation settings m/z tolerance (ppm): 3 Analysis version: Original MSM Pixel count: 469910 Imzml file size: 696.23 MB Ibd file size: 814.11 MB