M/Z: 470.0825

Hit 2 annotations: 2-(a-Hydroxyethyl)thiamine diphosphate_[M+H]+; 3,4,10,11-tetramethoxy-5-(trichloromethyl)-7,8-dihydro-5h-6-azatetraphene_[M+H]+

在BioDeep NovoCell知识数据库中，参考离子总共被划分为4个级别。

Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
Unreliable: 这个参考离子具有较高的排名价值，但缺乏可重复性。
Unavailable: 由于排名价值低且缺乏可重复性，这个参考离子不应用于注释。

Found 5 Reference Ions Near m/z 470.0825

NovoCell ID	m/z	Mass Window	Metabolite	Ranking	Anatomy Context
MSI_000017410 Reliable	470.0776	470.0776 ~ 470.0776 MzDiff: `0.0 ppm`	3,4,10,11-tetramethoxy-5-(trichloromethyl)-7,8-dihydro-5h-6-azatetraphene (BioDeep_00002095890) Formula: C22H22Cl3NO4 (469.0614)	2.48 (100%)	Vitis vinifera [PO:0009087] mesocarp
MSI_000016823 Unavailable	470.0776	470.0776 ~ 470.0776 MzDiff: `0.0 ppm`	3,4,10,11-tetramethoxy-5-(trichloromethyl)-7,8-dihydro-5h-6-azatetraphene (BioDeep_00002095890) Formula: C22H22Cl3NO4 (469.0614)	-0.06 (100%)	Vitis vinifera [PO:0009086] endocarp
MSI_000004618 Unreliable	470.0769	470.0769 ~ 470.0769 MzDiff: `none`	2-(a-Hydroxyethyl)thiamine diphosphate (BioDeep_00000005723) Formula: C14H23N4O8P2S+ (469.0712)	0.39 (100%)	Homo sapiens [UBERON:0002107] liver
MSI_000008771	470.0825	470.0825 ~ 470.0825 MzDiff: `none`	2-(a-Hydroxyethyl)thiamine diphosphate (BioDeep_00000005723) Formula: C14H23N4O8P2S+ (469.0712)	1.97 (100%)	Rattus norvegicus [UBERON:0004360] cauda epididymis
MSI_000024927 Unreliable	470.0922	470.0922 ~ 470.0922 MzDiff: `none`	(beta-1-O-[N-(2-hydroxymethyl-3-chlorophenyl)anthraniloyl]-D-glucupyranuronic acid) (BioDeep_00000053994) Formula: C20H20ClNO10 (469.0776)	1.75 (100%)	Mus musculus [UBERON:0004269] upper arm connective tissue

Found 8 Sample Hits

Metabolite	Species	Sample
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 8.6)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_43 Resolution: 17μm, 298x106 Description
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 8.6)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_44 Resolution: 17μm, 299x111 Description
3,4,10,11-tetramethoxy-5-(trichloromethyl)-7,8-dihydro-5h-6-azatetraphene Formula: C22H22Cl3NO4 (469.0614) Adducts: [M+H]+ (Ppm: 18.9)	Vitis vinifera (Fruit)	grape_dhb_164_1 Resolution: 17μm, 136x122 Description Grape berries fruit, condition: Late
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 8.8)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito08_48 Resolution: 17μm, 294x107 Description
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 8.8)	Rattus norvegicus (Epididymis)	epik_dhb_head_ito01_03 Resolution: 17μm, 159x110 Description
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 4.4)	Mus musculus (Lung)	image3 Resolution: 40μm, 146x190 Description Fig. 4 MALDI-MSI data of mouse lung tissue after administration with D9-choline and U13C-DPPC–containing Poractant alfa surfactant (labels administered 12 h prior to tissue collection). Ion images of (A) m/z 796.6856 ([U13C-DPPC+Na]+), (B) m/z 756.5154 [PC32:0+Na]+), and (C) m/z 765.6079 ([D9-PC32:0+Na]+). D: Overlay image of [U13C-PC32:0+Na]+ (red) and [D9-PC32:0+Na]+ (green). Part-per-million (ppm) mass errors are indicated in parentheses. All images were visualized using total-ion-current normalization and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0. MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC.
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 4.2)	Mus musculus (Lung)	image4 Resolution: 40μm, 162x156 Description Fig 6c Fig. 6 MALDI-MSI of U13C-PC16:0/16:0 acyl chain remodeling. A: Averaged MALDI mass spectrum from lung tissue collected from mice euthanized 12 h after administration of D9-choline and U13C-DPPC–containing Poractant alfa surfactant. The ion at m/z 828.6321 is assigned as the [M+Na]+ ion of 13C24-PC16:0_20:4 formed by acyl remodeling of U13C-PC16:0/16:0. The “NL” value refers to the intensity of the base peak in the full range MS1 spectrum. B: MS/MS spectrum of precursor ions at m/z 828.5 ± 0.5 with fragment ions originating from [13C24-PC16:0_20:4+Na]+ annotated. Part-per-million (ppm) mass errors are provided in parentheses. C, D: MALDI-MSI data of [U13C-DPPC+Na]+ (blue), [PC36:4+Na]+ (green) and [13C24-PC16:0_20:4+Na]+ (red) in lung tissue collected from mice (C) 12 h and (D) 18 h after label administration. All images were visualized using total-ion-current normalization and hotspot removal (high quantile = 99%). MS/MS, tandem mass spectrometry; MSI, mass spectrometry imaging; PC, phosphatidylcholine; U13C-DPPC, universally 13C-labeled dipalmitoyl PC.
2-(a-Hydroxyethyl)thiamine diphosphate Formula: C14H23N4O8P2S+ (469.0712) Adducts: [M+H]+ (Ppm: 2.7)	Mus musculus (Lung)	image5 Resolution: 40μm, 163x183 Description Supplementary Figure S8. MALDI-MSI data of mouse lung tissue administered with D9-choline and U 13C-DPPC–containing Poractant alfa surfactant (labels administered 18 h prior to sacrifice). Ion images of (a) m/z 796.6856 ([U13C-DPPC+Na]+), (b) m/z 756.5154 [PC32:0+Na]+ and (c) m/z 765.6079 ([D9-PC32:0+Na]+). (d) Overlay image of [U13C-DPPC+Na]+ (red) and [D9-PC32:0+Na]+ (green). Parts per million (ppm) mass errors are indicated in parentheses. All images were visualised using totalion-current normalisation and using hotspot removal (high quantile = 99%). DPPC = PC16:0/16:0.