在BioDeep NovoCell知识数据库中,参考离子总共被划分为4个级别。
  • Confirmed: 这个参考离子已经通过手动审计得到确认和验证。
  • Reliable: 这个参考离子可能在特定的解剖组织环境中高度保守。
  • Unreliable: 这个参考离子具有较高的排名价值,但缺乏可重复性。
  • Unavailable: 由于排名价值低且缺乏可重复性,这个参考离子不应用于注释。

Found 3 Reference Ions Near m/z 333.0475
NovoCell ID m/z Mass Window Metabolite Ranking Anatomy Context
MSI_000018190 Reliable 333.0475 333.0475 ~ 333.0475
MzDiff: 0.2 ppm
[(1-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]sulfanyl}ethylidene)amino]oxysulfonic acid (BioDeep_00002210042)
Formula: C8H15NO9S2 (333.0188)
0.28 (100%) Vitis vinifera
[PO:0009087] mesocarp
MSI_000016453 Unreliable 333.0475 333.0475 ~ 333.0475
MzDiff: 0.2 ppm
[(1-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]sulfanyl}ethylidene)amino]oxysulfonic acid (BioDeep_00002210042)
Formula: C8H15NO9S2 (333.0188)
0.94 (100%) Vitis vinifera
[PO:0009086] endocarp
MSI_000032169 Unreliable 333.0525 333.0525 ~ 333.0525
MzDiff: none
3,13-dihydroxy-6-methyl-9-(λ⁵-diazynylidene)-5-oxapentacyclo[8.8.0.0²,⁸.0⁴,⁶.0¹²,¹⁷]octadeca-1(10),2(8),12,14,16-pentaene-7,11,18-trione (BioDeep_00002157686)
Formula: C18H10N2O6 (350.0539)
2.31 (100%) Posidonia oceanica
[PO:0005020] vascular bundle

Found 6 Sample Hits
Metabolite Species Sample
Dbd-cocl

Formula: C11H13ClN4O4S (332.0346)
Adducts: [M+H]+ (Ppm: 11.8)
Marker Pen (NA)
3ul_0.8Mpa_RAW_20241016-PAPER PNMK
Resolution: 30μm, 315x42

Description

By writing the four English letters “PNMK” on white paper with a marker pen, and then scanning with a DESI ion source to obtain the scanning result. The signal of the chemical substances on the marker pen used appears on the channel with an m/z value of 322.1918, 323.1953, 546.4010, and etc, from the single cell deconvolution sampling layer class_4. This test data was tested by chuxiaoping from PANOMIX’s R&D laboratory.

[(1-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]sulfanyl}ethylidene)amino]oxysulfonic acid

Formula: C8H15NO9S2 (333.0188)
Adducts: [M-H2O+NH4]+ (Ppm: 16.3)
Vitis vinifera (Fruit)
grape_dhb_91_1
Resolution: 50μm, 120x114

Description

Grape berries fruit, condition: Ripe

[(1-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]sulfanyl}ethylidene)amino]oxysulfonic acid

Formula: C8H15NO9S2 (333.0188)
Adducts: [M-H2O+NH4]+ (Ppm: 16.3)
Vitis vinifera (Fruit)
grape_dhb_164_1
Resolution: 17μm, 136x122

Description

Grape berries fruit, condition: Late

[(1-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]sulfanyl}ethylidene)amino]oxysulfonic acid

Formula: C8H15NO9S2 (333.0188)
Adducts: [M-H2O+NH4]+ (Ppm: 16.3)
Vitis vinifera (Fruit)
grape_dhb_163_1
Resolution: 17μm, 132x115

Description

Grape berries fruit, condition: Late

3,13-dihydroxy-6-methyl-9-(λ⁵-diazynylidene)-5-oxapentacyclo[8.8.0.0²,⁸.0⁴,⁶.0¹²,¹⁷]octadeca-1(10),2(8),12,14,16-pentaene-7,11,18-trione

Formula: C18H10N2O6 (350.0539)
Adducts: [M+H-H2O]+ (Ppm: 5.7)
Posidonia oceanica (root)
20190614_MS1_A19r-20
Resolution: 17μm, 262x276

Description

Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth. Despite covering less than 0.1% of coastal regions, they have the capacity to bury up to 10% of marine organic matter and can bury the same amount of carbon 35 times faster than tropical rainforests. On land, the soil’s ability to sequestrate carbon is intimately linked to microbial metabolism. Despite the growing attention to the link between plant production, microbial communities, and the carbon cycle in terrestrial ecosystems, these processes remain enigmatic in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do not fully use this sugar stock in their metabolism. Instead, sucrose piles up in the sediments to mM concentrations underneath multiple types of seagrass meadows. Sediment incubation experiments show that microbial communities living underneath a meadow use sucrose at low metabolic rates. Our metagenomic analyses revealed that the distinct community of microorganisms occurring underneath meadows is limited in their ability to degrade simple sugars, which allows these compounds to persist in the environment over relatively long periods of time. Our findings reveal how seagrasses form blue carbon stocks despite the relatively small area they occupy. Unfortunately, anthropogenic disturbances are threatening the long-term persistence of seagrass meadows. Given that these sediments contain a large stock of sugars that heterotopic bacteria can degrade, it is even more important to protect these ecosystems from degradation.

1,2-Benzisothiazole-2(3H)-acetamide, N-(4-hydroxyphenyl)-3-oxo-, 1,1-dioxide

Formula: C15H12N2O5S (332.0467)
Adducts: [M+H]+ (Ppm: 0.5)
Homo sapiens (colorectal adenocarcinoma)
80TopL, 50TopR, 70BottomL, 60BottomR-profile
Resolution: 17μm, 137x136

Description

The human colorectal adenocarcinoma sample was excised during a surgical operation performed at the Imperial College Healthcare NHS Trust. The sample and procedures were carried out in accordance with ethical approval (14/EE/0024).