Metabolite annotation of rat epididymis with High-resolution imaging mass spectrometry (MTBLS58)

DOI: -
创建时间: 2025-01-01 02:06:06

Infertility or subfertility is a widespread problem with approximately 25% of couples seeking medical help. For about half the cases it lies in the male partner. Male infertility is caused by problems that affect sperm production, maturation and/or transport. In this study the role played by the epididymis in the post-testicular maturation of spermatozoa during its transit through this organ was investigated using FT-ICR imaging mass spectrometry of metabolites and the FDR-controlled metabolite annotation platform MetaSpace for high-resolution imaging mass spectrometry. This study together with MTBLS313 provides the accompanying data for FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry.

人工注释组织解剖术语列表
cauda epididymis, corpus epididymis, caput epididymis
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当前项目中的样本列表

epik_dhb_head_ito01_03

创建时间: 2025-01-02 22:49:41
空间分辨率: 17μm,   159x110
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito01_04

创建时间: 2025-01-02 21:43:24
空间分辨率: 17μm,   178x91
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito01_05

创建时间: 2025-01-02 23:50:39
空间分辨率: 17μm,   183x105
扫描: MALDI (DHB)
物种: Rattus norvegicus (normal) - normal

Note

epik_dhb_head_ito01_06

创建时间: 2025-01-03 01:24:14
空间分辨率: 17μm,   183x103
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito03_14

创建时间: 2025-01-03 08:32:25
空间分辨率: 17μm,   205x103
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito03_17

创建时间: 2025-01-01 02:06:06
空间分辨率: 17μm,   208x108
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note 1 male adult wild-type rat was obtained from Inserm U1085 - Irset Research Institute (University of Rennes1, France). Animals were age 60 days and were reared under ad-lib conditions. Care and handling of all animals complied with EU directive 2010/63/EU on the protection of animals used for scientific purposes. The whole epididymis was excised from each animal immediately post-mortem, loosely wrapped rapidly in an aluminum foil and a 2.5% (w/v) carboxymethylcellulose (CMC) solution was poured to embed the epididymis to preserve their morphology. To remove air bubbles, the filled aluminum molds was gently freezed by depositing it on isopentane or dry ice, then on the nitrogen vapors and finally by progressively dipping the CMC/sample coated with aluminum foil into liquid nitrogen (or only flush with liquid nitrogen). Frozen tissues were stored at -80 °C until use to avoid degradation.

epik_dhb_head_ito03_18

创建时间: 2025-01-01 02:30:51
空间分辨率: 17μm,   208x104
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito08_43

创建时间: 2025-01-01 14:32:10
空间分辨率: 17μm,   298x106
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito08_44

创建时间: 2025-01-01 15:43:45
空间分辨率: 17μm,   299x111
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito08_46

创建时间: 2025-01-01 17:25:08
空间分辨率: 17μm,   298x106
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito08_47

创建时间: 2025-01-02 14:31:37
空间分辨率: 17μm,   301x111
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note

epik_dhb_head_ito08_48

创建时间: 2025-01-02 17:29:41
空间分辨率: 17μm,   294x107
扫描: MALDI (DHB)
物种: Rattus norvegicus (Epididymis) - normal

Note