FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry (MTBLS313)

DOI: 10.1038/nmeth.4072
创建时间: 2025-01-22 11:21:41

High-mass-resolution imaging mass spectrometry promises to localize hundreds of metabolites in tissues, cell cultures, and agar plates with cellular resolution, but it is hampered by the lack of bioinformatics tools for automated metabolite identification. We report pySM, a framework for false discovery rate (FDR)-controlled metabolite annotation at the level of the molecular sum formula, for high-mass-resolution imaging mass spectrometry (https://github.com/alexandrovteam/pySM). We introduce a metabolite-signal match score and a target–decoy FDR estimate for spatial metabolomics.

人工注释组织解剖术语列表

neocortex, hippocampal formation, brainstem, cerebral cortex

Cite as:
Palmer, A., Phapale, P., Chernyavsky, I. et al. FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry. Nat Methods 14, 57–60 (2017). https://doi.org/10.1038/nmeth.4072

FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry (MTBLS313)

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Brain02_Bregma-3-88

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