Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves (MTBLS2960)

DOI: 10.7717/peerj-achem.21
创建时间: 2025-01-10 13:07:22

Lipids are an integral part of cellular membranes, vary between species, and allow cells to alter stiffness, permeability and curvature. Among lipids, phosphonolipids uniquely contain a phosphonate bond between carbon and phosphorous. Despite this distinctive biochemical characteristic, few models explore the biological role of phosphonolipids. A protective function has been inferred based on chemical and biological stability. The function of a tissue ultimately depends on its chemical makeup. Thus if the function of a tissue is known, for example, the barrier function of exposed epithelial cells, the function of an associated metabolite can be inferred. To investigate possible functions of phosphonolipids we analyzed two species of marine mollusks, the blue mussel Mytilus edulis and pacific oyster Crassostrea gigas, and determined the diversity of phosphonolipids and their distribution in different organs. High resolution spatial metabolomics revealed that the lipidome varies significantly between tissues within one organ. Despite their chemical similarity, the heterogeneity of the phosphonolipids within the lipid landscape likely reflects a functional diversity of the tissue. While some phosphonolipids are ubiquitously distributed, others are present almost exclusively in the ciliated epithelial cells. The exclusive localization of certain phosphonolipids in tissues exposed to the environment supports the hypothesis of a protective function in mollusks. This study highlights that the tissue specific distribution of an individual metabolite can be a valuable tool in inferring its function.

人工注释组织解剖术语列表
blank, gill ray, gill filament
Cite as:
Bourceau P, Michellod D, Geier B, Liebeke M. 2022. Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves. PeerJ Analytical Chemistry 4:e21 https://doi.org/10.7717/peerj-achem.21

当前项目中的样本列表

20190201_MS38_Crassostrea_Mantle_350-1500_DHB_pos_A28_10um_270x210

创建时间: 2025-01-10 13:07:22
空间分辨率: 10μm,   270x210
扫描: MALDI (DHB)
物种: Mytilus edulis (mantle) - normal

Note

20190202_MS38_Crassostrea_Gill_350-1500_DHB_pos_A25_11um_305x210

创建时间: 2025-01-10 14:03:21
空间分辨率: 11μm,   305x210
扫描: MALDI (DHB)
物种: Mytilus edulis (gill) - normal

Note single cell layer class_4 is the gill structure cells, metabolite ion 534.2956 is the top representive ion of this type of cell

20190216_MS38_Mytilus_mantle_350-1500_DHB_pos_A26_10um_275x210

创建时间: 2025-01-10 15:47:17
空间分辨率: 10μm,   275x210
扫描: MALDI (DHB)
物种: Mytilus edulis (mantle) - normal

Note