Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging (PXD001283)

DOI: 10.1002/anie.200905559
创建时间: 2024-12-27 12:44:33

https://onlinelibrary.wiley.com/doi/10.1002/anie.200905559

人工注释组织解剖术语列表

epithelial cell, muscle layer of urinary bladder, urinary bladder urothelium

Cite as:
Römpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B. Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl. 2010 May 17;49(22):3834-8. doi: 10.1002/anie.200905559. PMID: 20397170.

当前项目中的样本列表

HR2MSI_mouse_urinary_bladder - S096

创建时间: 2024-12-27 12:44:33
空间分辨率: 10μm, 260x134
扫描: MALDI (CHCA)
物种: Mus musculus (Urinary bladder) - normal

Note

Mass spectrometry imaging of phospholipids in mouse urinary bladder (imzML dataset)
The spatial distribution of phospholipids in a tissue section of mouse urinary bladder was analyzed by MALDI MS imaging at 10 micrometer pixel size with high mass resolution (using an LTQ Orbitrap mass spectrometer).

R, ö, mpp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B, Histology by mass spectrometry: label-free tissue characterization obtained from high-accuracy bioanalytical imaging. Angew Chem Int Ed Engl, 49(22):3834-8(2010)

Fig. S2: Single ion images of compounds shown in Fig. 1A-B : (upper left to lower right) m/z = 743.5482 (unknown), m/z = 741.5307 (SM (16:0), [M+K]+), m/z = 798.5410 (PC (34:1), [M+K]+), m/z = 616.1767 (heme b, M+), m/z = 772.5253 (PC (32:0), [M+K]+).

Stability of determined mass values was in the range of +/- 1 ppm over 22 hours of measurement (Fig. S4), with a standard deviation of 0.56 ppm. Accuracy data were obtained during tissue scanning experiments by monitoring the mass signal at nominal mass 798. The internal lock mass function of the Orbitrap instrument was used for automatic calibration during imaging measurements, using the known matrix-related ion signals at m/z = 137.0233, m/z = 444.0925 and m/z = 716.1246.